Abstract: Objective：To prepare a novel poly (lactic-co-glycolic acid) (PLGA) microcapsule scaffold for bone tissue engineering, characterize the scaffold, and detect its influence on the proliferation and adhesion of osteoblasts. Methods：PLGA was used to prepare microcapsules by double emulsion method. After analyzing the size distribution of PLGA microcapsules by the Malvern Mastersizer 2000, we sieved the microspheres into different sizes. Then we used ethylene chloride vapor to make the random packing of PLGA microcapsules with appropriate size combined. Further sintering helped us to create a three-dimensional microcapsule scaffold. The structure of microcapsule scaffolds were examined by scanning electron microscopy.Osteoblast cell line MC3T3-E1 was chosen to seed on the scaffold, and its adhesive status was observed by SEM. CCK-8 kit was used to analyze the cell proliferation of the group with scaffolds, and to compare with the group without scaffolds. Results：SEM showed that PLGA scaffolds has porous structure, with a certain pore size distribution and porosity. The in vitro study demonstrated that excellent cellular attachment throughout the microcapsule scaffolds. The results of CCK-8 demonstrated the cell viability of cells cultured on PLGA microcapsule scaffolds was higher than the control group after 9 days. Conclusions：From the above results we can conclude that the microcapsule-based scaffold has certain porosity and internal connectivity, which contributes to cell adhesion and proliferation. That means it may be promising as polymeric substitutes for bone repair.