实验性大鼠根尖周炎骨质破坏与RANKL/OPG关系的研究

›› 2013, Vol. 4 ›› Issue (4): 194-198.

实验性大鼠根尖周炎骨质破坏与RANKL/OPG关系的研究

王珏¹,吴叶²,曹灵³,袁俊¹,梅予锋⁴

1. 江苏省口腔医院
2. 南京医科大学附属南京医院
3. 南京医科大学口腔医学研究所
4. 南京医科大学

收稿日期:2013-09-11 修回日期:2013-12-12 出版日期:2013-12-25 发布日期:2014-01-09
通讯作者: 梅予锋 E-mail:yfmei@njmu.edu.cn
基金资助:
南京医科大学科技发展基金重点项目

The research of relationship between rats periapical bone destruction and RANKL / OPG system

1. stomatological Hospital of Jiangsu Province
2. Affiliated Nanjing Hospital of Nanjing Medical University
3. 缺
4. Stomotological Hospital of Jiangsu Provice
5.

Received:2013-09-11 Revised:2013-12-12 Online:2013-12-25 Published:2014-01-09

摘要/Abstract

摘要： 目的：观察核因子-κB受体活化因子配体（receptor activator for nuclear factor-κB ligand，RANKL）、骨保护素（osteoprotegerin，OPG）在大鼠根尖周炎发展各阶段表达的变化与根尖周病损发展之间的关系。方法：选取8周龄雄性Wistar大鼠，随机一侧下颌第一磨牙为实验组，对侧下颌第一磨牙作为对照组；术后1、3、7、14、21 d处死动物，拍摄颌骨X线片，计算根尖周骨破坏面积；组织切片，免疫组织化学检测根尖周组织中RANKL/OPG的表达。结果：根尖周骨破坏面积自术后3 d开始显著增大，21 d达到峰值，呈时间依赖关系；RANKL的表达量在3 d组明显增高，7 d组表达量到达峰值，14 d组开始下降；OPG的表达量在1 d组即出现有显著性上升，3 d组表达趋于平缓，21 d组达峰值。RANKL、OPG的变化与根尖周病变发展的变化趋势有着显著相关性。结论：RANKL/OPG系统在根尖周炎炎症性骨质破坏机制中起重要调节作用。

Abstract: Objective: The purpose of this study was to observe the immunohistochemical localization of RANKL(receptor activator for nuclear factor-κB ligand) and OPG(osteoprotegerin) and bone resorption in induced periapical lesions in rats.Methods: 8 -week-old male Wistar rats were use in this study, and those whose one side of their mandibular first molar pulp cavity was exposed to the oral cavity served as the treated group and those of the other side as control. Animals were randomly sacrificed at 1st day, 3rd day, 7th day, 14th day and 21st day after operation. We removed the jaw of rats, took X- ray to observe the apical lesion and sections were prepared for immunohistochemistry to detect RANKL / OPG expression in various stages of periapical tissue. Results: Rats periapical bone lesion area significantly increased from the 3rd day, reaching the peak at 21st day, which was time-dependent. RANKL expression of 3rd day group dramatically increased, reached the peak at 7th day, and began to decrease at 14th day. OPG expression sharply increased at 1st day and smoothly rose after 3rd days until it reached the peak at 21st days. The study found a significant correlation between the expression of RANKL/OPG system and the development of periapical lesions. Conclusions: RANKL / OPG system plays an important regulatory role in periapical inflammatory bone destruction.

王珏吴叶曹灵袁俊梅予锋. 实验性大鼠根尖周炎骨质破坏与RANKL/OPG关系的研究[J]. , 2013, 4(4): 194-198.

参考文献

[1]顾明燕, 毛慧忠, 张娟, 等.大鼠实验性根尖周炎中肿瘤坏死因子α的免疫组化测定[J].口腔医学,2008,28(5):253-255.
[2]Matsuo K, Irie N.Osteoclast-osteoblast communication[J].Arch Biochem Biophys,2008,473(2):201-209.
[3]Novack DV.Estrogen and bone: osteoclasts take center stage[J].Cell Metab,2007 ,6(4):254-256.
[4]Almeida M, Han L, Martin-Millan M, et al.Oxidative stress antagonizes Wnt signaling in osteoblast precursors by diverting beta-catenin from T cell factor- to forkhead box O-mediated transcription[J].J Biol Chem,2007,282(37):27298-27305.
[5]Hu JP, Nishishita K, Sakai E, et al.Berberine inhibits RANKL-induced osteoclast formation and survival through suppressing the NF-kappaB and Akt pathways[J].Eur J Pharmacol,2008,580(1/2):70-79.
[6]Galli C, Zella LA, Fretz JA, et al.Targeted deletion of a distant transcriptional enhancer of the receptor activator of nuclear factor-kappaB ligand gene reduces bone remodeling and increases bone mass[J].Endocrinology,2008,149(1):146-153.
[7]Qiu SX, Dan C, Ding LS, et al.A triterpene glycoside from black cohosh that inhibits osteoclastogenesis by modulating RANKL and TNFalpha signaling pathways[J].Chem Biol,2007,14(7):860-869.
[8]Wilson M, Reddi K, Henderson B.Cytokine-inducing components of periodontopathogenic bacteria[J].J Periodontal Res,1996,31(6):393-407.
[9]Teles R, Wang CY, Stashenko P.Increased susceptibility of RAG-2 SCID mice to dissemination of endodontic infections[J].Infect Immun,1997,65(9):3781-3787.
[10]Lin SK, Hong CY, Chang HH, et al.Immunolocalization of macrophages and transforming growth factor-beta 1 in induced rat periapical lesions[J].J Endod,2000,26(6):335-340.
[11]梅陵宣，刘正，张濒.实验性鼠根尖周炎组织学动态观察[J].实用口腔医学杂志,2002,18(6):500-503.
[12]Maruyama K, Takada Y, Ray N, et al.Receptor activator of NF-kappa B ligand and osteoprotegerin regulate proinflammatory cytokine production in mice[J].J Immunol,2006 ,177(6):3799-3805.