持续性根尖周炎根尖区微生物群落多样性分析

›› 2014, Vol. 5 ›› Issue (3): 120-124.

持续性根尖周炎根尖区微生物群落多样性分析

周耀,王娟

南京医科大学附属口腔医院

收稿日期:2014-08-14 修回日期:2014-08-27 出版日期:2014-09-25 发布日期:2014-09-24
通讯作者: 王娟 E-mail:candy1249@126.com
基金资助:
国家自然科学基金青年基金;江苏省高校自然科学研究项目;江苏高校优势学科建设工程资助项目

Bacterial flora associated with the apical segment of teeth with persistent periapical periodontitis

Affiliated Hospital of Stomatology, Nanjing Medical University

Received:2014-08-14 Revised:2014-08-27 Online:2014-09-25 Published:2014-09-24

摘要/Abstract

摘要： 目的：了解持续性根尖周炎患牙根尖段微生物群落的组成和多样性。方法：在根尖外科手术过程中采集13例持续性根尖周炎患牙根尖段样本，提取样本中微生物群落的总DNA。采用聚合酶链式反应-变性梯度凝胶电泳（polymerase chain reaction-denaturing gradient gel electrophoresis，PCR-DGGE）技术分析其微生物群落组成的多样性。对DGGE图谱上的条带进行割胶测序。结果：根尖段微生物群落为多菌种组成的细菌群落。但各样本间的相似度不高，菌群组成存在一定的差异。从DGGE凝胶上共获得26条可辨认的条带，检出菌种包括兼性厌氧菌、专性厌氧菌和需氧菌。其中，口腔放线菌属和口腔丙酸菌属的检出率较高，分别是84.6%和61.5%。结论：持续性根尖周炎患牙根尖段微生物群落为由多菌种组成的细菌群落。口腔放线菌属和口腔丙酸菌属有可能是持续性根尖周炎的条件致病菌。

Abstract: Objective：The aims of this study were to investigate bacterial flora associated with the apical segment of teeth with persistent periapical periodontitis. Methods： Apical root samples from root-end surgery were collected from 13 teeth with persistent periapical periodontitis. The DNA of the bacteria in 13 samples was processed for ampli?cation via polymerase chain reaction and separated with denaturing gradient gel electrophoresis. Selected bands were excised from the gel and sequenced for identi?cation. Results： All samples presented with several bands (strong and weak), which indicates a polymicrobial community. In our research, 26 excised bands were selected to identify the primary community members. The isolated strains consisted of facultative anaerobic bacteria, obligate anaerobic bacteria and aerobic bacteria. The prevalence rates of Actinomyces sp. oral and Propionibacterium were highest (84.6% and 61.5%, respectively). Conclusions：Bacterial floras associated with the apical segment of teeth with persistent periapical periodontitis are polymicrobial community. Actinomyces sp. oral and Propionibacterium are likely to be important contributors to persistent periapical infection.

周耀王娟. 持续性根尖周炎根尖区微生物群落多样性分析[J]. , 2014, 5(3): 120-124.

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