舌鳞状细胞癌中肿瘤干细胞对放疗敏感性体外研究

›› 2014, Vol. 5 ›› Issue (3): 125-129.

舌鳞状细胞癌中肿瘤干细胞对放疗敏感性体外研究

杨雪超¹,吕孝帅¹,陆瑶伽²

1. 广州医科大学附属口腔医院
2. 浙江省嘉兴市第二人民医院口腔科

收稿日期:2014-08-18 修回日期:2014-09-11 出版日期:2014-09-25 发布日期:2014-09-24
通讯作者: 杨雪超 E-mail:xyang.gmu@gmail.com
基金资助:
广东省科技计划项目;广东省医学科研基金项目;广州市荔湾区科技计划项目

The radiosensitivity of cancer stem cells in squamous cell carcinoma of tongue

Received:2014-08-18 Revised:2014-09-11 Online:2014-09-25 Published:2014-09-24

摘要/Abstract

摘要： [摘要] 目的:探讨舌鳞状细胞癌中肿瘤干细胞对放疗的敏感性。方法:采用磁珠分选技术、细胞免疫荧光染色和细胞增殖活性试验等方法，研究经分选后得到的肿瘤干细胞和阴性细胞在放疗后其在细胞增殖活性方面的差异。结果:细胞免疫荧光结果显示，CD133和CD44在分选出来的CD133+/CD44+ 细胞高表达而在CD133-/CD44- 细胞基本不表达。CCK8实验结果显示分选出来的CD133+/CD44+ 细胞较CD133-/CD44- 细胞在放疗的作用下具有更高的细胞成活率。结论:经磁珠分选出来的舌癌肿瘤干细胞（CD133+/CD44+ 细胞）更能耐受放疗的作用。

Abstract: Objective： To investigate the radiosensitivity of cancer stem cells in squamous cell cacinoma of tongue. Methods：CD133+/CD44+ and CD133-/CD44- subpopulation cells were sorted by magnetic cell sorting (MACS). Single isolated CD133+/CD44+ and CD133-/CD44- cell was cultured in RPMI-1640 mediun，which contained 10 ng/mL basic fibroblast growth factor (bFGF)，and 20 ng/mL epidermal growth factor (EGF). Immunofluorescence was applied to detect the expression of CD133 and CD44 in these two subpopulation cells. CCK-8 assay was performed to analyze the radiosensitivity of the two subpopulations. Results：Cell immunofluorescence showed that CD133 and CD44 could be detected in CD133+/CD44+ cells while they could not be detected in CD133-/CD44- cells. Radiation therapy had a more obvious inhibitory effect on CD133-/CD44- cells than on CD133+/CD44+ cells. Conclusions： The cancer stem cells in squamous cell carcinoma of tongue(CD133+/CD44+ cells) that sorted by MACS showed strong capability of tumor's resistance to radiation therapy than that of CD133-/CD44- cells.

中图分类号:

R322.4

杨雪超吕孝帅陆瑶伽. 舌鳞状细胞癌中肿瘤干细胞对放疗敏感性体外研究[J]. , 2014, 5(3): 125-129.

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