4-硝基喹啉-1-氧化物诱导大鼠舌癌过程中Notch1的表达特点

›› 2014, Vol. 5 ›› Issue (3): 135-139.

4-硝基喹啉-1-氧化物诱导大鼠舌癌过程中Notch1的表达特点

谢友群¹,王钊¹,储伟明¹,张玮²,钟旖³,张玮⁴,祁兵³,王子露⁵,吴煜农⁶,宋晓萌⁵

1. 南京医科大学口腔研究所
2. 江苏省口腔医院口腔颌面外科
3. 江苏省口腔医院病理科
4. 江苏省口腔医院特诊科
5. 南京医科大学附属口腔医院
6. 江苏省口腔医院颌面外科

收稿日期:2014-07-21 修回日期:2014-08-28 出版日期:2014-09-25 发布日期:2014-09-24
通讯作者: 吴煜农 E-mail:wynoms@gmail.com

Study on the variation of Notch1 expression during the course of SD rats tongue carcinoma induced by 4-NQO

Received:2014-07-21 Revised:2014-08-28 Online:2014-09-25 Published:2014-09-24

摘要/Abstract

摘要： 目的：研究Notch1在大鼠舌癌模型发生发展过程中的表达变化。方法：将40只SD大鼠随机分为对照组（A组10只）和实验组（B、C、D组各10只），使用质量百分浓度为0.004%的4-硝基喹啉-1-氧化物（4-nitroquinoline-1-oxide，4NQO）饮用水喂养实验组SD大鼠，用蒸馏水喂养对照组SD大鼠。B、C、D组分别于8、16、24周取大鼠舌体组织；A组在8、16、24周同时各取3只、3只、4只作为正常对照。行常规组织学观察，并使用免疫组织化学法检测Notch1在病变不同阶段的舌粘膜内的表达。结果：4NQO可以有效诱导大鼠舌黏膜鳞状细胞癌的发生。在不同的给药时间点，可以诱导大鼠舌黏膜产生不同程度的癌前病变。Notch1在正常舌黏膜、癌前病变、舌鳞癌中的表达逐渐增高(P<0.01)。随着异常增生程度的增加，阳性表达部位逐渐由基底层向表层扩展，且Notch1的膜型表达逐渐增多。结论：Notch1的高表达及其在细胞膜上的集中表达可能与舌癌的发生、发展过程相关。

Abstract: Objective：To establish a tongue epithelial carcinoma model in Sprague-Dawley rats and to study the expression of Notch1 in different phases of carcinogenesis. Methods：A total of 40 Sprague-Dawley rats were divided into four groups: A, B, C and D. Rats in experimental groups B, C and D were fed with 0.004% 4-nitroquinoline-1-oxide (4-NQO) dissolved in drinking water for 8, 16 and 24 weeks, respectively; while rats in control group A were fed with normal distilled water. Rats in experimental groupB, C and D were sacrificed at 8, 16, 24 weeks, respectively, along with 3 rats from normal group A, simultaneously. The tongue samples were dissected and detached for HE staining and Notch1 immunohistochemistry studies. Results：Different pathological changes including mild dysplasia, moderate-to-severe dysplasia, in-situ carcinoma and invasive carcinoma were observed with the advance of administration time. Positive expression rate of Notch1 in normal mucosa, dysplasia, carcinoma were 20%(2/10), 64.7%(11/17) and 84.6%(11/13), respectively(P<0.01). In normal tongue mucosa, the positive staining of Notch1 was mainly distributed in stratum basale, while it extended from stratum basale to stratum corneum as the progression of carcinogenesis. Conclusions：Notch1 promotes the carcinogenesis of tongue mocosa and its expression on membrane may play a role in tongue carcinoma.

谢友群王钊储伟明张玮钟旖张玮祁兵王子露吴煜农宋晓萌. 4-硝基喹啉-1-氧化物诱导大鼠舌癌过程中Notch1的表达特点[J]. , 2014, 5(3): 135-139.

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