外源性拮抗神经纤毛蛋白1促进人舌癌细胞SCC9细胞凋亡的研究

›› 2014, Vol. 5 ›› Issue (4): 185-188.

外源性拮抗神经纤毛蛋白1促进人舌癌细胞SCC9细胞凋亡的研究

查霖¹,吴煜农²,郑颢¹,宋晓萌²,唐子春³

1. 铜陵市人民医院口腔科
2. 南京医科大学附属口腔医院
3. 安徽省铜陵市人民医院口腔科

收稿日期:2014-09-23 修回日期:2014-12-09 出版日期:2014-12-25 发布日期:2015-01-06
通讯作者: 唐子春 E-mail:tzc9439@126.com
基金资助:
安徽省铜陵市卫生局科研项目

Exogenous inhibition of Neuropilin-1(NRP-1) enhance the apoptosis of human tongue squamous cell carcinoma cell line SCC9

Received:2014-09-23 Revised:2014-12-09 Online:2014-12-25 Published:2015-01-06

摘要/Abstract

摘要： 目的：研究外源性拮抗人舌癌细胞SCC9中神经纤毛蛋白1（Neuropilin-1，NRP-1)蛋白对人舌癌细胞SCC9增殖、凋亡的影响。方法：通过免疫印迹实验（western blot，WB）及实时定量荧光PCR（real-time RT-PCR）分别从蛋白水平及mRNA水平检测小分子肽ATWLPPR（A7R）对SCC9细胞NRP-1表达的影响，利用细胞计数法及流式细胞术检测拮抗NRP-1蛋白后对SCC9细胞增殖、凋亡的影响。结果：A7R拮抗后SCC9细胞NRP-1的mRNA水平下降而蛋白表达未见明显下降，同时对SCC9细胞凋亡有促进作用，而对SCC9细胞增殖无明显影响。结论：外源性拮抗NRP-1可促进SCC9细胞凋亡。

Abstract: Objective: To investigate the effect on the proliferation and the apoptosis of the human tongue squamous carcinoma cell line SCC9 through exogenous inhibition of Neuropilin-1(NRP-1). Methods: Western blot and real-time RT-PCR were employed to examine the effect on the expression of NRP-1 in cell line SCC9 exogenous inhibition of NRP-1. Besides, cell counting and flow cytometry(FC) methods were used to examine the influence of the proliferation and the apoptosis of the SCC9 cells exogenous inhibition of NRP-1. Results: After the inhibition, mRNA level of NRP-1 decreased, whereas the expression of protein level of NRP-1 did not decrease obviously. Exogenous inhibition of NRP-1 enhances the apoptosis of SCC9 cells while rarely affects the proliferation of SCC9 cells. Conclusions: Exogenous inhibition of NRP-1 enhances the apoptosis of the SCC9 cells.

中图分类号:

R782

查霖吴煜农郑颢宋晓萌唐子春. 外源性拮抗神经纤毛蛋白1促进人舌癌细胞SCC9细胞凋亡的研究[J]. , 2014, 5(4): 185-188.

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