小鼠舌肌发育相关基因的功能聚类分析

›› 2015, Vol. 6 ›› Issue (2): 62-65.

小鼠舌肌发育相关基因的功能聚类分析

丛蔚¹,刘波¹,蒋玉玲²,肖晶¹

1. 大连医科大学口腔医学院口腔基础教研室
2. 大连医科大学口腔医学院, 口腔基础教研室

收稿日期:2015-03-27 修回日期:2015-05-17 出版日期:2015-06-25 发布日期:2015-07-13
通讯作者: 丛蔚 E-mail:congwei@dlmedu.edu.cn
基金资助:
国家自然科学基金;辽宁省自然科学基金

Functional annotation clustering of genes in mouse tongue myogenesis

1. Dalian Medical University
2. 大连医科大学口腔医学院口腔基础教研室
3. Department of Oral Basic Science, College of Stomatology, Dalian Medical University

Received:2015-03-27 Revised:2015-05-17 Online:2015-06-25 Published:2015-07-13

摘要/Abstract

摘要： 目的：研究小鼠舌肌发育的分子调控机制。方法：取胚胎第13.25天（E13.25）及E15.5小鼠舌组织。应用Affymetrix Mouse GeneChip，对胎鼠舌发育过程中的差异基因进行筛选。应用DAVID网络分析工具对基因进行功能和聚类分析。结果：基因功能和聚类分析表明，在E13.25高表达的基因主要与细胞周期相关因子（Exo1、Gsk3B、Kif20b、Skp2）和细胞粘附因子（Neo1、lama1）等相关。在E15.5高表达的基因主要与细胞骨架（titin、Hspb7）相关。结论：小鼠舌组织增殖和特化与细胞周期和细胞粘附基因相关，舌组织分化和成熟主要与细胞骨架相关。

Abstract: Objective：To gain insight into the molecular mechanisms associated with mouse tongue myogenesis. Methods：Different genes in the tongue at mouse embryonic day 13.25 (E13.25) and 15.5 was investigated using Affymetrix Mouse GeneChip. Using the twice significance of difference as the standard, the molecular mechanisms of tongue development were studied and several molecules related were identified by DAVID functional annotation clustering analysis. Results：Genes of higher expression level at E13.25 were related to cell cycle and cell adhesion, of whom Exo1, Gsk3B, Kif20b, Skp2 ( cell cycle related factors) and Neo1 and lama1 (cell adhesion factors) were activated. While genes of higher expression level at E15.5 were related to cytoskeleton, such as titin and Hspb7. Conclusions：The proliferation and determination of tongue were related with gene clusters of cell cycle and cell adhesion, and, differentiation and maturation of tongue were relevant to gene cluster of cytoskeleton. It had highlighted potential cascades and important candidates for further investigation on the genetic mechanism and clinical therapy of tongue related diseases.

丛蔚刘波蒋玉玲肖晶. 小鼠舌肌发育相关基因的功能聚类分析[J]. , 2015, 6(2): 62-65.

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