不同年龄段小鼠下颌前导后髁突骨骼改建差异的研究

›› 2016, Vol. 7 ›› Issue (1): 26-29.

不同年龄段小鼠下颌前导后髁突骨骼改建差异的研究

王龙凤¹,王冬玥¹,郭舒瑜¹,倪洁丽²,马俊青³,李琥⁴,邵胜⁴,林汤毅⁴,李媛⁴,侯伟⁴,刘翔⁵

1. 南京医科大学
2. 南京医科大学口腔医学研究所
3. 南京医科大学口腔医学院
4. 南京医科大学附属口腔医院
5. 南京医科大学口腔疾病研究江苏省重点实验室

收稿日期:2015-07-28 修回日期:2015-11-03 出版日期:2016-03-25 发布日期:2016-04-06
通讯作者: 马俊青 E-mail:majunq@163.com
基金资助:
国家自然科学基金;江苏高校优势学科建设工程资助项目

Expression of bone remodeling in mice condylar during mandibular advancement and the differences between different age

1. Nanjing Medical University
2. Institute of Stomatology, School of Stomatology, Nanjing Medical University.
3.
4. Institute of Stomatology，Nanjing Medical University
5. Insititute of Stomatology，School of Stomatology，Nanjing Medical University
6. Institute of Stomatology, Department of Orthodontics，Affiliated Hospital of Stomatology, Nanjing Medical University
7. Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University

Received:2015-07-28 Revised:2015-11-03 Online:2016-03-25 Published:2016-04-06

摘要/Abstract

摘要： 目的：观测小鼠间歇性下颌前导后髁状突的形态学变化，比较不同年龄段的组织改建差异，为下颌前导的矫治时机和策略提供理论依据。方法：在4周龄和8周龄BABL/c雌鼠上建立间歇性下颌前导模型，于下颌前导后的14 d和28 d取颞下颌关节标本行Trap染色、Godener三色染色、微型计算机断层扫描（Micro-CT）技术来研究髁突破骨细胞及成骨细胞变化和骨骼形态学变化。结果：28 d时4周龄组相对于28 d时8周龄组，骨体积分数（BV/TV）和骨小梁厚度（TB.Th）减小，骨小梁间距（TB.Sp）增大（P <0.05）。骨小梁数量（TB.N）无明显差异（P >0.05）。14 d时4周龄组相对于14 d时8周龄组以及14 d时4周龄组相对于28 d时4周龄组，每视野中破骨细胞和成骨细胞数升高（P <0.05）。结论：小鼠导下颌向前与髁突骨骼改建有密切联系，不同年龄段差异显著。

Abstract: Objective: To observe histomorphology changes of condyle and differences between young and adult through mandibular advancement intermittently so as to provide the opportunity and strategy of mandibular advancement. Methods: Mandibular advancement model was established on 4 weeks old and 8 weeks old BABL/c female rats. The TMJ samples were harvested at the 14th and 28th days and stored for Trap staining and Godener trichrome staining and Micro-CT scan. Results: Compared with those at 28th day of the 8-week-old group, the at 28th day of the 4-week-old group was as follows: BV/TV and TB.Th decreased; Tb.Sp increased(P<0.05); TB.N was not different(P>0.05). Compared with those at 14th day of the 8-week-old group, the at 14th day of the 4-week-old group was as follows: the number of Trap positive osteoclasts and the number of osteoblasts were all increased(P<0.05). Compared with those at 28th day of the 4-week-old group, the at 14th day of the 4-week-old group was as floows: the number of Trap positive osteoclasts and the number of osteoblasts were all increased(P<0.05). Conclusion: Bone remodeling on rats is related with mandibular advancement and the has significant differences between different ages.

中图分类号:

R783.9

王龙凤王冬玥郭舒瑜倪洁丽马俊青李琥邵胜林汤毅李媛侯伟刘翔. 不同年龄段小鼠下颌前导后髁突骨骼改建差异的研究[J]. , 2016, 7(1): 26-29.

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