内毒素耐受对THP-1细胞分泌ENA-78和EGF的影响

›› 2016, Vol. 7 ›› Issue (1): 30-33.

内毒素耐受对THP-1细胞分泌ENA-78和EGF的影响

祝祥清,周侨,陈杨,徐艳,孙颖

南京医科大学口腔医学院

收稿日期:2015-12-23 修回日期:2016-01-24 出版日期:2016-03-25 发布日期:2016-04-06
通讯作者: 孙颖 E-mail:ebolasun@163.com
基金资助:
国家自然科学基金;江苏省高等学校大学生实践创新训练计划;江苏高校优势学科建设工程资助项目

Effects of endotoxin tolerance on the production of ENA-78 and EGF in THP-1 cells

Received:2015-12-23 Revised:2016-01-24 Online:2016-03-25 Published:2016-04-06

摘要/Abstract

摘要： 目的：观察脂多糖（lipopolysaccharides，LPS）诱导的内毒素耐受对人单核细胞株THP-1细胞分泌趋化因子上皮来源的中性粒细胞活化肽78（epithelial neutrophil-activating peptide 78，ENA-78）和表皮生长因子（epidermal growth factor，EGF）的影响。方法：采用1μg/ml牙龈卟啉单胞菌（Porphyromonas gingivalis, P. gingivalis）LPS或1μg/ml大肠杆菌（Escherichia coli，E.coli）LPS分别刺激THP-1细胞24h，洗脱后，再次刺激24h，诱导细胞产生耐受。采用ELISA技术检测ENA-78和EGF表达水平的变化。结果：P. gingivalis LPS单次刺激后，ENA-78水平与刺激前无明显差别（p>0.05），E.coli LPS单次刺激后，ENA-78水平较刺激前明显增高（p<0.05）；两种LPS重复刺激后，ENA-78水平均较单次刺激后明显降低（p<0.05）。但两种LPS单次/重复刺激后，EGF分泌水平均无明显变化（p>0.05）。结论：内毒素耐受能抑制THP-1细胞分泌ENA-78，进而可能对中性粒细胞趋化产生影响。

Abstract: Objective：To observe the effects of endotoxin tolerance on the production of chemokine epithelial neutrophil-activating peptide 78 (ENA-78) and epidermal growth factor (EGF) in THP-1 cells. Methods：THP-1 cells were pretreated with 1μg/ml Porphyromonas gingivalis (P.gingivalis) lipopolysaccharides (LPS) or 1μg/ml Escherichia coli (E.coli) LPS (24h), washed and stimulated with the same LPS again (24h). Levels of ENA-78 and EGF in supernatants were detected by ELISA. Results：There were no differences in production of ENA-78 secreted by THP-1 cells stimulated with P.gingivalis LPS and culture medium (p>0.05), while the amounts of ENA-78 in the cells challenged by E.coli LPS were increased significantly compared with those without any stimulations (p<0.05). In addition, secretions of ENA-78 were decreased significantly after repeated P.gingivalis LPS or E.coli LPS stimulations compared with those following signal challenge (p<0.05). However, no significant changes of EGF levels were detected in THP-1 cells restimulated with P.gingivalis LPS or E.coli LPS compared with those challenged only once (p>0.05). Conclusions：Secretions of ENA-78 in THP-1 cells could be suppressed by endotoxin tolerance, which might have an effect on neutrophil migration.

祝祥清周侨陈杨徐艳孙颖. 内毒素耐受对THP-1细胞分泌ENA-78和EGF的影响[J]. , 2016, 7(1): 30-33.

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