TNF-α抑制miR-21影响小鼠骨髓基质干细胞成骨分化

›› 2016, Vol. 7 ›› Issue (2): 67-71.

TNF-α抑制miR-21影响小鼠骨髓基质干细胞成骨分化

王光¹,杨楠²,丁寅³

1. 解放军91551门诊部
2. 解放军第309医院口腔科
3. 第四军医大学口腔医院正畸科

收稿日期:2016-04-07 修回日期:2016-05-25 出版日期:2016-06-25 发布日期:2016-06-22
通讯作者: 丁寅 E-mail:dingyin@fmmu.edu.cn

TNF-α inhibits mBMSCs osteoporosis through miR-21

Received:2016-04-07 Revised:2016-05-25 Online:2016-06-25 Published:2016-06-22

摘要/Abstract

摘要： 目的：探讨肿瘤坏死因子-α（TNF-α）是否能通过微小RNA（miR）-21影响小鼠骨髓基质干细胞（mBMSCs）的成骨分化。方法：利用实时定量RT-PCR检测TNF-α对miR-21的影响。在实时定量RT-PCR、Western blot等技术验证TNF-α对mBMSCs成骨能力的影响后，通过转染技术在含有TNF-α的成骨环境下上调miR-21的表达，进而观察转染后mBMSCs的成骨能力。结果：TNF-α抑制mBMSCs成骨分化，转染miR-21至含有TNF-α的成骨环境下的mBMSCs能部分恢复其成骨分化能力。结论：miR-21介导的成骨分化是TNF-α抑制mBMSCs成骨的途径之一。

Abstract: Objective：To investigate whether or not the TNF-α can affect mouse bone marrow stromal stem cells (mBMSCs)osteoporosis through miR-21.Methods：Real-time RT-PCR was used to detect the levels of miR-21 in mBMSCs osteogenesis on condition of TNF-α. Osteogenic gene and protein was determined by real-time RT-PCR and Western blot analysis, respectively. After upregulation the of miR-21 in the presence of TNF-α, the osteogenic potential of mBMSCs were investigated. Results：TNF-α inhibits mBMSCs osteoporosis and miR-21 can restore the potential of mBMSCs treated with TNF-α. Conclusions：TNF-α inhibits mBMSCs osteoporosis through miR-21.

王光杨楠丁寅. TNF-α抑制miR-21影响小鼠骨髓基质干细胞成骨分化[J]. , 2016, 7(2): 67-71.

参考文献

[1]Passeri G, Girasole G, Jilka RL，et al.Increased interleukin-6 production by murine bone marrow and bone cells after estrogen withdrawal[J].Endocrinology, 1993, 133(2):822-828
[2]O'Brien CA, Gubrij I, Lin SC, et al.STAT3 activation in stromal/osteoblastic cells is required for induction of the receptor activator of NF-kappaB ligand and stimulation of osteoclastogenesis by gp130-utilizing cytokines or interleukin-1 but not 1,25-dihydroxyvitamin D3 or parathyroid hormone[J].J Biol Chem, 1999, 274(27):19301-19308
[3]Deswal A, Petersen NJ, Feldman AM,.Cytokines and cytokine receptors in advanced heart failure: an analysis of the cytokine database from the Vesnarinone trial (VEST)[J].Circulation, 2001, 103(16):2055-2059
[4]Rifas L, Arackal S.T cells regulate the of matrix metalloproteinase in human osteoblasts via a dual mitogen-activated protein kinase mechanism[J].Arthritis Rheum, 2003, 48(4):993-1001
[5]Teitelbaum SL.Postmenopausal osteoporosis, T cells, and immune dysfunction[J].Proc Natl Acad Sci U S A, 2004, 101(48):16711-16712
[6]Li YP, Stashenko P.Proinflammatory cytokines tumor necrosis factor-alpha and IL-6, but not IL-1, down-regulate the osteocalcin gene promoter[J].J Immunol, 1992, 148(3):788-794
[7]王光，杨楠，丁寅，等.MiR-21调控雌激素缺乏导致的小鼠骨质疏松BMSCs成骨能力的研究[J].口腔生物医学, 2015, 6(3):137-142
[8]Gilbert L, He X, Farmer P, et al.Inhibition of osteoblast differentiation by tumor necrosis factor-alpha[J].Endocrinology, 2000, 141(11):3956-3964
[9]Lacey DC, Simmons PJ, Graves SE,.Proinflammatory cytokines inhibit osteogenic differentiation from stem cells: implications for bone repair during inflammation[J].Osteoarthritis Cartilage, 2009, 17(6):735-742
[10]Zhang B, Pan X, Anderson TA.MicroRNA: a new player in stem cells[J].J Cell Physiol, 2006, 209(2):266-269
[11]Taganov KD, Boldin MP, Chang KJ,et al.NF-kappaB-dependent induction of microRNA miR-146, an inhibitor targeted to signaling proteins of innate immune responses[J].Proc Natl Acad Sci U S A, 2006, 103(33):12481-12486
[12]Rodríguez JP, Garat S, Gajardo H, et al.Abnormal osteogenesis in osteoporotic patients is reflected by altered mesenchymal stem cells dynamics[J].J Cell Biochem, 1999, 75(3):414-423
[13]Ohno M, Natsume A, Kondo Y, et al.The modulation of microRNAs by type I IFN through the activation of signal transducers and activators of transcription 3 in human glioma[J].Mol Cancer Res, 2009, 7(12):2022-2030
[14]Gilbert L, He X, Farmer P, et al.Inhibition of osteoblast differentiation by tumor necrosis factor-alpha[J].Endocrinology, 2000, 141(11):3956-3964
[15]Chang J, Wang Z, Tang E,.Inhibition of osteoblastic bone formation by nuclear factor-kappaB[J].Nat Med, 2009, 15(6):682-689
[16]Gilbert L, He X, Farmer P,.Expression of the osteoblast differentiation factor RUNX2 (Cbfa1/AML3/Pebp2alpha A) is inhibited by tumor necrosis factor-alpha[J].J Biol Chem, 2002, 277(4):2695-2701