Abstract: Objective：To develop an animal model of infantile hemangioma by applying conditional transgenic mice. Methods：pTie2-PyMT, a plasmid of Tie2 promoter-driven polyomavirus middle T (PyMT) gene, was constructed. Endothelial specific PyMT gene was transported to male pronucleus of donor C57BL/6Jmice by DNA micro-injection, and the fertilized eggs were transplanted to receptor mice. The founder mice were developed from the surviving fertilized eggs. The gene integration was checked by PCR, and phenotypes of transgenic mice were examined. The neoplasms of transgenic mice were detected by histological method. GraphPad Prism 5.0 software package was used for statistical analysis. Results：The PyMT, Tie2 promoter and Tie2 enhancer were cloned and integrated exactly in pTie2-PyMT plasmid according to sequencing analysis. All the newborn positive transgenic mice had hemangioma phenotypes. The hemangioma-like neoplasms were identified by HE in the ear, tongue, skin surface, mucosa and liver tissue in the transgenic mice, and immunohistology staining showed positive of PyMT and CD31 in endothelial cells. Conclusions：PyMT gene driven by Tie2 promoter can induce hemangioma in mice. Thus, the transgenic mice can be applied as a model system for studying the mechanism of infantile hemangioma. Conditional transgenic technology is an ideal method to develop hemangioma animal model.