甲状旁腺激素对去势大鼠骨髓间充质干细胞增殖及凋亡的影响

›› 2016, Vol. 7 ›› Issue (3): 122-128.

甲状旁腺激素对去势大鼠骨髓间充质干细胞增殖及凋亡的影响

轩诗杰¹,曹猛¹,杨振华¹,刘竹影²,杨璇璇¹,卢晓琳²,丁寅²

1. 第四军医大学口腔医院正畸科，军事口腔医学国家重点实验室
2. 第四军医大学口腔医院正畸科

收稿日期:2016-06-28 修回日期:2016-08-31 出版日期:2016-09-25 发布日期:2016-09-28
通讯作者: 丁寅 E-mail:dingyin@fmmu.edu.cn
基金资助:
Periostin促进骨质疏松症牙周组织再生修复作用的研究

The effects of parathyroid hormone on proliferation and apoptosis ability of bone marrow mesenchymal stem cells in ovariectomized rats

Received:2016-06-28 Revised:2016-08-31 Online:2016-09-25 Published:2016-09-28

摘要/Abstract

摘要： 目的：研究甲状旁腺激素（PTH）对去势大鼠骨髓间充质干细胞增殖以及凋亡特性的影响。方法：建立去势大鼠骨质疏松模型，皮下注射甲状旁腺激素，通过流式细胞仪技术检测去势大鼠骨髓间充质干细胞细胞周期以及凋亡率的改变。结果：加PTH刺激大鼠骨髓间充质干细胞组的S期细胞比例为（8.87±0.11）%，显著高于去势大鼠骨髓间充质干细胞组的S期细胞比例（5.23±0.20）%，差异具有统计学意义（P<0.05）；加PTH刺激大鼠骨髓间充质干细胞组凋亡率为（4.17±0.15）%，显著低于去势大鼠骨髓间充质干细胞组凋亡率（8.13±0.61）%，差异具有统计学意义（P<0.05）。结论：甲状旁腺激素可明显提高去势大鼠骨髓间充质干细胞的增殖能力，并抑制其凋亡。

Abstract: Objective：To investigate the effects of parathyroid hormone (PTH) on proliferation and apoptosis ability of bone marrow mesenchymal stem cells (BMMSCs) from ovariectomized（OVX）rats. Methods：An ovariectomized animal model wasadopted. The rats were treated with either subcutaneous PTH or normal saline. The proliferation and apoptosis of BMMSCs were detected by means of flow cytometry. Results:The S-phase fraction of BMMSC in ovariectomized with PTH treatment group （8.87±0.11）% was significantly increased when compared with ovariectomized group（5.23±0.20）%. And the apoptosis of BMMSC in ovariectomized with PTH treatment group（4.17±0.15）% was significantly reduced when compared with ovariectomized group（8.13±0.61）%. Conclusions：Intermittent application of PTH can strengthen the proliferation ability of BMMSCs from OVXrats but suppress the apoptosis.

轩诗杰曹猛杨振华刘竹影杨璇璇卢晓琳丁寅. 甲状旁腺激素对去势大鼠骨髓间充质干细胞增殖及凋亡的影响[J]. , 2016, 7(3): 122-128.

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