Abstract: Objective： We designed a novel strategy for enamel remineralization, including an amelogenin-like oligopeptide as templates for controlling the nucleation and growth of HA crystals and synthesized calcium- and phosphate-loaded temperature sensitive liposomes supplying calcium and phosphate ion, to induce HA crystals and promote biomimetic mineralization. Methods：A novel polyanion amelogenin-inspired oligropeptide (Gln-Pro-Ala)4-Thr-Lys-Arg-Glu-Glu-Val-Asp) was synthesized by standard solid-phase, and induced self-assembly with CaCl2 solution. Secondly, the calcium/phosphate phosphate loaded liposomes were prepared using the interdigitation-fusion method. The liposomes were composed of 1,2-bis(palmitoyl)-sn-glycero-3-phosphocholine and 1,2-bis(myristoyl)-sn-glycero-3-phosphocholine. Lastly, the experimental groups treated with mixture solution of liposomes and polyanion peptide. But the control groups were treated with the liposomes solution. These two groups were characterized by scanning electron microscope(SEM), Fourier transform infrared spectroscopy(FTIR) and X-ray Diffraction(XRD). Results：The experimental groups showed that the rod-like crystals almost fully covered the etched enamel surface, and distributed evenly to make the surface smooth. In contrast, the control groups showed that there was only small amount of HA crystal deposition, and the precipitate distributed randomly. Conclusions：The self-assembly amelogenin-inspired oligopeptide polyanion peptide combining with temperature sensitive Ca2+ and PO43- loaded liposomes can effectively induce biomimetic mineralization of enamel.