miR-223调控人牙周膜成纤维细胞中RANKL表达的功能初探

›› 2016, Vol. 7 ›› Issue (4): 183-186.

miR-223调控人牙周膜成纤维细胞中RANKL表达的功能初探

杨迷芳¹,王珏²,马兰³,谢理哲²,于金华⁴

1. 南京医科大学口腔医学研究所
2. 南京医科大学
3. 南京医科大学口腔疾病研究江苏省重点实验室
4. 南京医科大学口腔医学院

收稿日期:2016-12-08 修回日期:2016-12-23 出版日期:2016-12-25 发布日期:2017-01-09
通讯作者: 杨迷芳 E-mail:yangmifang@qq.com

Investigation of miR-223 on the of RANKL in human periodontal ligament fibroblasts cells

Received:2016-12-08 Revised:2016-12-23 Online:2016-12-25 Published:2017-01-09
Contact: Mi-Fang Yang E-mail:yangmifang@qq.com

摘要/Abstract

摘要： 目的：探讨miR-223对人牙周膜成纤维细胞中RANKL的调控作用。方法：构建过表达miR-223的人牙周膜成纤维细胞，western blot及real time PCR检测人牙周膜成纤维细胞内RANKL、OPG的表达，计算RANKL/OPG比值的改变。结果：过表达miR-223的人牙周膜成纤维细胞， RANKL的表达明显下调，RANKL/OPG比值下降。结论：miR-223可调控RANKL的表达，破坏RANKL/OPG比例的平衡，改变破骨细胞的微环境，影响破骨细胞分化。

Abstract: Objective： Investigate the role of miR-223 on the of RANKL in HPDLFs. Methods：The HPDLFs over expressed with miR-223 was constructed, the of RANKL and OPG were detected by western blot and real time PCR, and the change of RANKL/OPG ratio was calculated. Results: The RANKL was down-regulated in the HPDLFs with miR-223 over expressed and the RANKL/OPG ratio was decreased. Conclusions: MiR-223 could disrupt the homeostasis of RANKL/OPG, destroy the microenvironment of osteoclast differentiation. MiR-223 may play a regulatory role in osteoclast differentiation .

杨迷芳王珏马兰谢理哲于金华. miR-223调控人牙周膜成纤维细胞中RANKL表达的功能初探[J]. , 2016, 7(4): 183-186.

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