牙龈卟啉单胞菌脂多糖对成骨细胞EphA4基因表达的调控

›› 2017, Vol. 8 ›› Issue (1): 25-27.

牙龈卟啉单胞菌脂多糖对成骨细胞EphA4基因表达的调控

刘歆婵¹,侯玉帛²,王柳然²,于维先²,宫琳²,周延民²

1. 吉林大学口腔医学院种植中心
2. 吉林大学口腔医院

收稿日期:2016-12-13 修回日期:2017-02-24 出版日期:2017-03-25 发布日期:2017-04-11
通讯作者: 刘歆婵 E-mail:1028606641@qq.com
基金资助:
国家自然科学基金面上项目;吉林省科技厅自然科学基金项目

Effect of Porphyromonas gingivalis lipopolysaccharide on the of EphA4 gene in ST-2 Cells

1. Department of Dental Implantology, School and Hospital of Stomatology, Jilin University
2. Stomatological Hospital, Jilin University

Received:2016-12-13 Revised:2017-02-24 Online:2017-03-25 Published:2017-04-11

摘要/Abstract

摘要： 目的：研究牙龈卟啉单胞菌脂多糖（Pg-LPS）对小鼠骨髓基质细胞系ST-2在成骨分化过程中Eph受体酪氨酸激酶A4基因（EphA4）表达的影响。方法：使用终浓度10 μg/mL的Pg-LPS与ST-2细胞共培养，分别在培养第1、3和7天，采用RT-PCR技术检测EphA4基因及Runt相关转录因子2（Runx2）Runx2、Ⅰ型胶原蛋白（ColⅠ）和碱性磷酸酶（ALP）等成骨相关基因的表达。结果：实验组EphA4基因表达在培养第1、3和7天时比对照组分别减少2.5、2.4和2.3倍，两组之间存在显著差异（P＜0.01）；实验组Runx2基因表达在培养第1、3天比对照组减少，两组之间存在显著差异（P＜0.01），但在第7天两组表达均不明显；实验组ColⅠ和ALP基因表达在培养第1、3和7天时均比对照组减少，差异有统计学意义（P＜0.01）。结论：Pg-LPS能抑制ST-2细胞成骨分化过程中EphA4基因的表达，同时也抑制成骨相关基因Runx2、ColⅠ和ALP的表达。

Abstract: Objective：To observe the effect of Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) on the of EphA4 in mouse marrow stromal cell line ST-2. Methods：ST-2 cells were co-cultured with 10 μg/mL of Pg-LPS at 1 d, 3 d and 7 d. EphA4 gene and the osteogenic related genes (Runx2, ColⅠ and ALP) were detected by RT-PCR. Results：Compared with Pg-LPS group, at the 1 d, 3 d and 7 d, the EphA4 mRNA was significantly decreased by 2.5 folds，2.4 folds and 2.3 folds in control group. At the same time，the osteogenic related gene Runx2 in the experimental group were significantly decreased (P < 0.01) as compared with that of the control group at 1 d and 3 d, but on the 7 d this was not obvious; as to the osteogenic related genes ColⅠand ALP s in 1 d, 3 d and 7 d, the experimental group was less than the control group, and the difference was statistically significant (P<0.01). Conclusions：Pg-LPS can inhibit the s of EphA4 gene and osteogenic related genes Runx2, ColⅠand ALP in ST-2 cells in the osteogenetic differentiation.

中图分类号:

R781.4

刘歆婵侯玉帛王柳然于维先宫琳周延民. 牙龈卟啉单胞菌脂多糖对成骨细胞EphA4基因表达的调控[J]. , 2017, 8(1): 25-27.

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