氟环境下变形链球菌耐氟菌株rpl基因的差异表达

›› 2017, Vol. 8 ›› Issue (1): 28-31.

氟环境下变形链球菌耐氟菌株rpl基因的差异表达

张琦¹,赵洪岩¹,张志民²,张红¹,李文月¹,超博¹,杨瑶瑶¹

1. 吉林大学口腔医院牙体牙髓病科
2. 吉林大学口腔医学院

收稿日期:2016-12-21 修回日期:2017-02-06 出版日期:2017-03-25 发布日期:2017-04-11
通讯作者: 张志民 E-mail:zhangzm1964@sina.com
基金资助:
吉林省科技厅自然科学基金

Differential of rpl gene in fluoride-resistant Streptococcus mutans cultivated in fluoride environment

Received:2016-12-21 Revised:2017-02-06 Online:2017-03-25 Published:2017-04-11

摘要/Abstract

摘要： 目的：检测并分析变形链球菌耐氟菌株在氟环境下培养时rpl基因表达的改变。方法：分别将变形链球菌亲代菌株及耐氟菌株置于无氟脑心浸液（BHI）培养基，另将耐氟菌株置于含1g/L NaF的BHI培养基中，均培养至11 h（对数生长期）、20 h（稳定生长期）后提取总RNA并逆转录，采用实时定量RT-PCR技术检测各组变形链球菌rpl基因的表达。结果：与无氟培养比较，高氟培养的耐氟菌株rpl基因表达在对数期无差异（P＞0.05），而在稳定期表达升高（P<0.001）；与亲代菌株比较，耐氟菌株在无氟及高氟培养基中rpl的表达量均明显下降（P<0.001）。结论：氟可以增加稳定生长期的变形链球菌耐氟菌株rpl基因的表达。

Abstract: Objective：To determine whether there is significant difference in of rpl gene of fluoride-resistant Streptococcus mutans cultivated in fluorine-containing medium. Methods：Fluoride-resistant Streptococcus mutans were subcultured in BHI containing 1g/L NaF or without NaF, and its parental strain was subcultured in BHI without NaF. After 11h (logarithmic phase) or 20h (platform stage) cultivation, total RNA of the three groups were extracted and reverse transcribed，and the impression level of rpl were detected by qRT-PCR. Results：The of rpl gene of fluoride-resistant Streptococcus mutans cultivated in fluorine-containing medium, compared with fluoride-free medium, had no significant difference in the logarithmic phase (P＞0.05), but increased in the platform stage (P<0.001). Compared with its parental strain, the of rpl gene of fluoride-resistant Streptococcus mutans, whether cultivated in fluorine-containing or fluorine-free medium, was significantly decreased (P<0.001). Conclusions：The presence of fluoride could weaken the decrease of the of rpl gene in fluoride-resistant Streptococcus mutans, compared with its parental strain, in the platform stage, but had no influence in the logarithmic phase.

张琦赵洪岩张志民张红李文月超博杨瑶瑶. 氟环境下变形链球菌耐氟菌株rpl基因的差异表达[J]. , 2017, 8(1): 28-31.

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