Abstract: Objective：To prepare light cured polyurethane acrylate adhesive for dentin bonding and we focus on its water absorption/solubility and in vitro cytotoxicity. Methods：Polyether and polyester blend polyurethane has been prepared with solution polymerization process, along with methyl methacrylate (MMA), butyl acrylate (BAM) as diluent preparation to obtain the polyurethane acrylate adhesive (PU) used in the dentin bonding. Then we evaluated water absorption/solubility and cytotoxicity of PU adhesive, and compared with two commercial adhesives（Single Bond2,Adper Easy One）. Results：water absorption: PU<Adper Easy One<Single Bond 2，water solubility: PU< Single Bond2 <Adper Easy One；In MTT assay，cytotoxicity of PU adhesive and two commercial adhesives exposed to human pulp cells was PU<Adper Easy One<Single Bond 2。Cytotoxicity was affected by the exposure time, 24 h<48 h<72 h. Conclusions：PU adhesive shows good resistance to hydrolysis performance and good biocompatibility compared with the traditional acrylic ester resin adhesive.