白藜芦醇促进氧化应激状态牙髓干细胞在钛种植体表面的增殖及成骨分化

›› 2017, Vol. 8 ›› Issue (3): 155-158.

白藜芦醇促进氧化应激状态牙髓干细胞在钛种植体表面的增殖及成骨分化

郭英¹,张静莹²,曲哲¹,张乃馨²,Kenny Man³,杨学斌³

1. 大连市口腔医院
2. 大连大学医学院
3. 利兹大学

收稿日期:2017-06-28 修回日期:2017-08-11 出版日期:2017-09-25 发布日期:2017-10-24
通讯作者: 曲哲 E-mail:quzhekq@outlook.com
基金资助:
国家自然科学基金;辽宁省自然科学基金

Effects of resveratrol on proliferation and osteogenic differentiation of dental pulp stem cells on titanium implants in oxidative stress

Received:2017-06-28 Revised:2017-08-11 Online:2017-09-25 Published:2017-10-24

摘要/Abstract

摘要： 目的：观察白藜芦醇（RSV）对氧化应激状态人源牙髓干细胞（hDPSCs）在大颗粒喷砂酸蚀表面改性的钛片表面增殖及成骨分化的影响。方法：采用酶消化法体外分离培养hDPSCs，200 μmol/L H2O2作用24 h诱导hDPSCs产生氧化应激状态，10 μmol/L RSV处理氧化应激状态的hDPSCs 24 h；AlamerBlue法观察hDPSCs增殖活性的改变，扫描电镜观察hDPSCs在钛表面黏附及形态的改变，碱性磷酸酶（ALP）染色观察hDPSCs在钛片表面成骨分化能力的改变。结果：氧化应激状态的hDPSCs增殖能力比正常对照组显著降低，RSV能够显著提高氧化应激状态hDPSCs的增殖能力，并能促进其在钛片表面ALP的表达。结论：RSV能够促进氧化应激状态的hDPSCs在钛片表面增殖及成骨分化。

Abstract: Objective：To observe the effects of resveratrol on the proliferation and osteogenic differentiation of human dental pulp stem cells (hDPSCs) modified by SLA on oxidized stress. Methods：Enzyme digestion was used to isolate cultured human dental pulp derived hDPSCs in vitro. Oxidative stress was induced by hydrogen peroxide (200 μmol/L) for 24 hours. Application of 10 μmol/L resveratrol to hDPSCs was treated with hydrogen peroxide. The effects of resveratrol on the proliferation of hDPSCs after oxidative stress was observed by AlamerBlue method. The effects of resveratrol on the adhesion and morphology of hDPSCs on SLA titanium surface were observed by scanning electron microscopy. The effect of resveratrol on the osteogenic differentiation of hDPSCs on oxidized stress was observed by ALP staining. Results：The proliferation ability of hDPSCs of oxidative stress was significantly lower than that of the normal control group. Resveratrol could significantly increase the proliferation of hDPSCs of oxidative stress and promote the adhesion and morphology change of hDPSCs and the ALP expression on the surface of SLA modified. Conclusions：Resveratrol could promote the proliferation and bone differentiation ability of hDPSCs on the surface of SLA modified titanium.

郭英张静莹曲哲张乃馨 Kenny Man 杨学斌. 白藜芦醇促进氧化应激状态牙髓干细胞在钛种植体表面的增殖及成骨分化[J]. , 2017, 8(3): 155-158.

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