变形链球菌氟抗性相关蛋白EriCF1和EriCF2研究进展

摘要/Abstract

摘要： 变形链球菌是目前公认的致龋力最强、检出率最高的细菌之一。氟化物被用于龋病的预防和治疗，既能保护牙体硬组织又能抑制变形链球菌等致龋菌的生长和代谢。由于氟的广泛使用，出现了耐氟菌株的选择性生长。对变形链球菌氟抗性机制的研究，有助于人们进一步了解变形链球菌氟抗性的产生和发展。本文在蛋白和基因两个层面对变形链球菌及其耐氟菌株氟抗性主要机制—氟离子运输蛋白EriCF1和EriCF2研究进展加以综述，旨在为新型分子药物的研制指明方向，也为龋病的预防和治疗提供重要的理论基础。

Abstract: Streptococcus mutans with the highest detection rate is recognized as one of the most cariogenic bacteria at present.Fluoride is used for the prevention and treatment of caries,which can protect the hard tissues of the teeth and inhibit the growth and metabolism of cariogenic bacteria,such as Streptococcus mutans.Because of the wide application of fluorine, the selective growth of fluoride resistant bacteria has been observed. The research on the mechanism of fluorosis resistance of Streptococcus mutans will help us further understand the generation and development of fluorosis resistance in Streptococcus mutans.In this paper, we summarize the research progress of fluoride transporter EriCF1 and EriCF2, the main mechanism of fluoride resistance in Streptococcus mutans and its fluoride resistant bacteria on the two aspects of protein and gene.The purpose of this review is to provide a direction for the development of new molecular drugs and to provide an important theoretical basis for the prevention and treatment of caries.?

中图分类号:

R780.2

曲添张红张志民张志鹰. 变形链球菌氟抗性相关蛋白EriCF1和EriCF2研究进展[J]. , 2018, 9(2): 102-105.

参考文献

[1]Brown L R, White J O, Horton I M, et al.Effect of continuous fluoride gel use on plaque fluoride retention and microbial activity[J].Journal of Dental Research, 1983, 62(6):746-746
[2]Buzalaf M A, Pessan J P, Honório H M, et al.Mechanisms of action of fluoride for caries control[J][J].Monographs in Oral Science, 2011, 22(20):97-114
[3]Ten Cate JM (2004) Fluorides in caries prevention and control: empiricism or science.Fluorides in Caries Prevention and Control: Empiricism or Science[J].Caries Research, 2004, 38(3):254-257
[4]Huang F, Haydock S F, Spiteller D, et al.The Gene Cluster for Fluorometabolite Biosynthesis in Streptomyces cattleya: A Thioesterase Confers Resistance to Fluoroacetyl-Coenzyme A[J].Chemistry & Biology, 2006, 13(5):475-84
[5]Li S, Smith K D, Davis J H, et al.Eukaryotic resistance to fluoride toxicity mediated by a widespread family of fluoride export proteins[J].Proceedings of the National Academy of Sciences of the United States of America, 2013, 110(47):19018-23
[6]Stockbridge R B, Lim H H, Otten R, et al.Fluoride resistance and transport by riboswitch-controlled CLC antiporters[J].Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(38):15289-15294
[7]Baker J L, Sudarsan N, Weinberg Z, et al.Widespread genetic switches and toxicity resistance proteins for fluoride[J].Science, 2012, 335(6065):233-5
[8]刘丽慧, 田健, 伍宁丰.细菌氟抗性研究进展[J].中国农业科技导报, 2013, 15(6):113-118
[9]Nelson J, Plummer M, Blount K, et al.Small Molecule Fluoride Toxicity Agonists[J].Chemistry & Biology, 2015, 22(4):527-527
[10]Matsui R, Cvitkovitch D.Acid tolerance mechanisms utilized by Streptococcus mutans[J].Future Microbiology, 2010, 5(3):403-417
[11]Ji C, Stockbridge R B, Miller C.Bacterial fluoride resistance,Fluc channels,and the weak acid accumulation effect[J].Journal of General Physiology, 2014, 144(3):257-61
[12]Stockbridge R B, Robertson J L, Kolmakovapartensky L, et al.A family of fluoride-specific ion channels with dual-topology architecture[J].Elife Sciences, 2013, 2(200):e01084-e01084
[13]Ames T D, Rodionov D A, Weinberg Z, et al.A Eubacterial Riboswitch Class That Senses the Coenzyme Tetrahydrofolate[J].Chemistry & Biology, 2010, 17(7):681-5
[14]Ren A, Rajashankar K R, Patel D J.Fluoride ion encapsulation by Mg2+ and phosphates in a fluoride riboswitch[J].Nature, 2012, 486(7401):85-9
[15]Maduke M, Pheasant D J, Miller C.High-level expression, functional reconstitution, and quaternary structure of a prokaryotic ClC-type chloride channelen[J].Journal of General Physiology, 1999, 114(5):713-713
[16]Breaker R R.New insight on the response of bacteria to fluoride[J].Caries Research, 2012, 46(1):78-81
[17]Streckfuss J L, Perkins D, Horton I M, et al.Fluoride resistance and adherence of selected strains of Streptococcus mutans to smooth surfaces after exposure to fluoride[J].Journal of Dental Research, 1980, 59(2):151-8
[18]Brussock S M, Kral T A.Effects of pH on expression of sodium fluoride resistance in Streptococcus mutans[J].Journal of Dental Research, 1987, 66(10):1594-1594
[19]Murata T, Hanada N.Contribution of chloride channel permease to fluoride resistance in Streptococcus mutans[J].Fems Microbiology Letters, 2016, 363(11):f-n
[20]Accardi A, Picollo A.CLC channels and transporters: Proteins with borderline personalities[J].[J]. Biochim Biophys Acta. 2010, 1798(8):1457-1464., 2010, 1798(8):1457-1464
[21]Accardi A, Miller C.Secondary active transport mediated by a prokaryotic homologue of ClC Cl- channels[J].Nature, 2004, 427(6977):803-803
[22]Liao Y, Chen J, Brandt B W, et al.Identification and Functional Analysis of Genome Mutations in a Fluoride-Resistant Streptococcus mutans Strain[J].Plos One, 2015, 10(4):e0122630-e0122630
[23]Stockbridge R B, Kolmakova-Partensky L, Shane T, et al.Crystal structures of a double-barrelled fluoride ion channel[J].Nature, 2015, 525(7570):548-548
[24]Baker J L, Sudarsan N, Weinberg Z, et al.Widespread genetic switches and toxicity resistance proteins for fluoride[J].Science, 2012, 335(6065):233-5
[25]Alessandra P, Xu Y, Niklaus J, et al.Synergistic substrate binding determines the stoichiometry of transport of a prokaryotic H+:Cl?exchanger[J].Nature Structural & Molecular Biology, 2012, 19(5):525-S1
[26]Ji C, Stockbridge R B, Miller C.Bacterial fluoride resistance,Fluc channels,and the weak acid accumulation effect[J].Journal of General Physiology, 2014, 144(3):257-61
[27]Men X, Shibata Y, Takeshita T, et al.., 2016, 11(11).[J]. Plos One, 2016, 11(11):e0165900-e0165900
[28]Leenhouts K, Buist G, Bolhuis A, et al.A general system for generating unlabelled gene replacements in bacterial chromosomes[J].Molecular & General Genetics Mgg, 1996, 253(1-2):217-224
[29]Ying L, Brandt B W, Min Z, et al.A Single Nucleotide Change in the Promoter mutp Enhances Fluoride Resistance of Streptococcus mutans[J].Antimicrobial Agents & Chemotherapy, 2016, 60(12):7509-7509
[30]Zhu L, Zhang Z, Liang J.Fatty-acid profiles and expression of the fabM gene in a fluoride-resistant strain of Streptococcus mutans[J].Archives of Oral Biology, 2012, 57(1):10-14
[31]Marquis R E, Clock S A, Motameira M.Fluoride and organic weak acids as modulators ofmicrobial physiology[J].Fems Microbiology Reviews, 2003, 26(5):493-493