SFRP2对小鼠淋巴结总T细胞的作用研究

›› 2019, Vol. 10 ›› Issue (2): 58-61.

• 论著 • 下一篇

SFRP2对小鼠淋巴结总T细胞的作用研究

梁运存¹,范志朋²

1. 首都医科大学附属北京口腔医院
2. 首都医科大学附属北京口腔医院研究所

收稿日期:2019-03-20 修回日期:2019-05-21 出版日期:2019-06-25 发布日期:2019-07-01
通讯作者: 范志朋 E-mail:zpfan@ccmu.edu.com

Effect of SFRP2 on the total T cells of lymph node from mouse

¹,

Received:2019-03-20 Revised:2019-05-21 Online:2019-06-25 Published:2019-07-01

摘要/Abstract

摘要： 目的：研究分泌型卷曲相关蛋白2（SFRP2）对C57小鼠淋巴结总T细胞功能的影响。方法：利用SFRP2重组蛋白构建高浓度SFRP2条件；体外分离培养C57小鼠淋巴结总T细胞；流式细胞术检测0.4 ng/mL SFRP2重组蛋白对C57小鼠淋巴结总T细胞的增殖、凋亡及亚群的影响。结果：0.4 ng/mL SFRP2重组蛋白抑制总T细胞的增殖和凋亡，主要抑制早期凋亡，对晚期凋亡率无明显影响；0.4 ng/mL SFRP2重组蛋白增加Th1亚群比例，降低Th2亚群比例，提高了Th1/Th2比例。结论：一定浓度SFRP2重组蛋白可以抑制总T细胞的增殖和凋亡，并促进T细胞向Th1亚群分化，抑制其向Th2亚群分化。

关键词: 分泌型卷曲相关蛋白2, 总T细胞, Th1/Th2平衡

Abstract: Objective： To study the effect of secreted frizzled-related protein 2 (SFRP2) on lymph node T cells in C57 mice. Methods： SFRP2 recombinant protein was used to construct high concentration SFRP2. The total T cells of lymph node from C57 mouse were isolated and cultured in vitro. The effects of SFRP2 recombinant protein on proliferation, apoptosis and subpopulation of lymph node T cells in C57 mice were detected by flow cytometry. Results： SFRP2 (0.4 ng/mL) inhibited the proliferation of total T cells;SFRP2 (0.4 ng/mL)inhibited the early apoptosis of total T cells, has no significant effect on late apoptosis rate, and inhibits total apoptosis rate; SFRP2 (0.4 ng/mL) increases the proportion of Th1 subpopulations, decreases the proportion of Th2 subpopulations, and changes the Th1/Th2 balance. Conclusions： The results indicate that the concentration of SFRP2 (0.4 ng/mL) can inhibit the proliferation of total T cells, promote apoptosis, and affect the balance of Th1/Th2.

Key words: secreted frizzled-related protein 2 , total T cell, Th1/Th2balance

梁运存范志朋. SFRP2对小鼠淋巴结总T细胞的作用研究[J]. , 2019, 10(2): 58-61.

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SFRP2对小鼠淋巴结总T细胞的作用研究

Effect of SFRP2 on the total T cells of lymph node from mouse

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