年龄因素对牙周膜干细胞多向分化能力的影响

›› 2019, Vol. 10 ›› Issue (2): 78-82.

年龄因素对牙周膜干细胞多向分化能力的影响

李晓钰¹,王红²,谷秀格¹,魏福兰²

1. 山东大学口腔医学院正畸科
2. 山东大学

收稿日期:2019-03-28 修回日期:2019-05-24 出版日期:2019-06-25 发布日期:2019-07-01
通讯作者: 魏福兰 E-mail:weifl@email.sdu.edu.cn
基金资助:
国家自然科学基金

The effect of aging on the multi-directional differentiation ability of human periodontal ligament stem cells

Received:2019-03-28 Revised:2019-05-24 Online:2019-06-25 Published:2019-07-01

摘要/Abstract

摘要： 目的：探讨年龄因素对人牙周膜干细胞（PDLSCs）多向分化能力的影响。方法：选取因阻生需要拔除的具有完整牙根的第三磨牙共14颗，根据患者年龄分为青少年组（16~19岁）和中老年组（38~50岁），分别刮取牙周膜组织，分离培养获得PDLSCs，用流式细胞术分析干细胞表面标记物；通过体外成骨、成脂诱导培养，比较两组PDLSCs的多向分化能力。结果：经过流式细胞术检测，两组PDLSCs均具有间充质干细胞的特性，但中老年组PDLSCs的成骨、成脂分化能力较青少年组明显减弱（P＜0.05）。结论：PDLSCs的多向分化能力随年龄的增加而降低。

关键词: 牙周膜干细胞, 年龄, 分化能力

Abstract: Objective： To investigate the effect of aging on the multi-directional differentiation ability of human periodontal ligament stem cells (PDLSCs). Methods： 14 healthy third molars were collected with complete roots in clinic and divided into two groups according to the donors’ ages: the younger group (donor age at 16-19 years) and the older group (donor age at 38-50 years). The periodontal ligament tissues were obtained and PDLSCs were isolated and cultured. Flow cytometry analysis was used to identify the phenotypes of PDLSCs. To compare the multi-directional differentiation ability of PDLSCs, cells were subjected to osteogenic and adipogenic induction in vitro respectively. Results： Flow cytometry analysis revealed that PDLSCs in both groups had the characteristics of mesenchymal stem cells. And the multi-directional differentiation ability of PDLSCs in the older group was significantly decreased compared with that in the younger group(P＜0.05). Conclusions： The multi-directional differentiation ability of PDLSCs decreased with age.

Key words: periodontal ligament stem cells , aging, multi-directional differentiation ability

李晓钰王红谷秀格魏福兰. 年龄因素对牙周膜干细胞多向分化能力的影响[J]. , 2019, 10(2): 78-82.

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