红景天苷对小鼠成牙本质细胞系MDPC-23增殖、迁移和矿化能力影响的研究

口腔生物医学 ›› 2024, Vol. 15 ›› Issue (5): 265-270.

红景天苷对小鼠成牙本质细胞系MDPC-23增殖、迁移和矿化能力影响的研究

关健¹,吕晶¹,高雪峰²,金星爱¹

1. 哈尔滨医科大学
2. 哈尔滨医科大学附属第一医院

收稿日期:2024-03-11 修回日期:2024-03-29 出版日期:2024-10-25 发布日期:2024-11-04
通讯作者: 金星爱 E-mail:1792704368@qq.com
基金资助:
黑龙江省教育厅科研项目面上项目

Study on the effect of salidroside on proliferation,migration and mineralization of mouse odontoblast cell line MDPC-23

Received:2024-03-11 Revised:2024-03-29 Online:2024-10-25 Published:2024-11-04

摘要/Abstract

摘要： 目的：探究红景天苷（SAL）对小鼠成牙本质细胞系MDPC-23增殖、迁移和矿化能力的影响。方法：体外培养小鼠成牙本质细胞系MDPC-23，用不同浓度SAL（0、10、20、50、100 μmol/L）培养MDPC-23，通过CCK-8法、活/死细胞染色法、划痕实验、Transwell实验检验SAL对MDPC-23毒性、增殖和迁移能力影响。对0、10、20 μmol/L SAL组MDPC-23进行矿化诱导培养，通过碱性磷酸酶（ALP）染色及活性检测、茜素红染色、钙结节定量测定、实时荧光定量PCR和Western blot实验评估各组细胞矿化能力。结果：SAL可促进细胞的增殖且无明显细胞毒性，以20 μmol/L效果最为显著（P＜0.05）；SAL对细胞迁移有显著促进作用（P＜0.05）。SAL诱导MDPC-23产生更多的矿化结节（P＜0.05）。20 μmol/L SAL显著促进ALP表达（P＜0.05）。SAL处理MDPC-23后的ALP、骨钙素（OCN）和Runt相关转录因子（Runx2）、牙本质涎磷蛋白（DSPP）以及牙本质基质蛋白-1（DMP-1）的基因表达显著增加（P＜0.05），OCN和Runx2蛋白表达水平显著增加（P＜0.05）。结论：SAL可促进MDPC-23的增殖、迁移和矿化。

关键词: 红景天苷, 小鼠成牙本质细胞系, 盖髓剂, 成骨/成牙本质分化

Abstract: Objective: To investigate the influence of salidroside (SAL) on the proliferation, migration and mineralization abilities of the mouse odontoblast cell line MDPC-23. Methods: MDPC-23 cells were cultured in vitro and stimulated with different concentrations of salidroside (0 μmol/L, 10 μmol/L, 20 μmol/L, 50 μmol/L and 100 μmol/L). The effects of salidroside on MDPC-23 proliferation and migration were examined by using CCK-8, live-dead cell staining, scratch assay and Transwell assay. Three groups were set up: control group (osteogenic medium), and 10μmol/L SAL group and 20 μmol/L SAL group. The mineralization capacity of SAL was evaluated by alkaline phosphatase (ALP) staining, ALP activity assay, alizarin red staining (ARS), calcium nodule quantification , qRT-PCR and western blot. Results: SAL promoted cell proliferation without significant cytotoxicity, with the most significant effect at 20 μmol/L (P＜0.05). SAL promoted cell proliferation across the experimental concentration range, with 20 μmol/L SAL exhibiting a significant proliferative effect. SAL had no significant cytotoxicity. SAL significantly enhanced cell migration (P＜0.05). SAL treatment resulted in a greater number of mineralized nodules (P＜0.05). ALP staining and activity assays indicated that 20 μmol/L SAL significantly promoted ALP expression (P＜0.05). The expression of ALP, osteocalcin (OCN), Runt-related transcription factor 2 (Runx2), dentin sialophosphoprotein (DSPP), and dentin matrix protein-1 (DMP-1) were significantly increased (P＜0.05), and the protein expression levels of OCN and Runx2 were also significantly increased after SAL treatment (P＜0.05). Conclusions: SAL can promote the proliferation, migration and mineralization abilities of odontoblast cell line MDPC-23 in mice.

Key words: salidroside, MDPC-23, pulp capping agent, osteogenic/odontogenic differentiation

关健吕晶高雪峰金星爱. 红景天苷对小鼠成牙本质细胞系MDPC-23增殖、迁移和矿化能力影响的研究[J]. 口腔生物医学, 2024, 15(5): 265-270.

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