siRNA干扰FOXM1基因对涎腺腺样囊性癌细胞增殖的影响

›› 2012, Vol. 3 ›› Issue (3): 120-123.

siRNA干扰FOXM1基因对涎腺腺样囊性癌细胞增殖的影响

丁旭¹,李怀奇¹,武和明²,吴煜农¹

1. 南京医科大学附属口腔医院
2. 南京医科大学附属口腔医院江苏省口腔医院口腔颌面外科

收稿日期:2012-08-03 修回日期:2012-08-16 出版日期:2012-09-25 发布日期:2012-10-08
通讯作者: 吴煜农 E-mail:yunongwu@yahoo.com

The effect of FOXM1 siRNA on proliferation of salivary adenoid cystic carcinoma cells

Received:2012-08-03 Revised:2012-08-16 Online:2012-09-25 Published:2012-10-08

摘要/Abstract

摘要： 目的：探讨FOXM1对涎腺腺样囊性癌细胞增殖的影响。方法：以高转移腺样囊性癌细胞系（SACC-M）为对象，构建FOXM1 siRNA干扰片段，瞬转SACC-M细胞，蛋白质免疫印迹法检测干扰效率；流式细胞仪分析FOXM1干扰后SACC-M细胞周期的改变，甲基噻唑基四唑比色法检测干扰后ACC-M细胞的增殖，蛋白质免疫印迹法检测干扰后细胞周期蛋白CyclinB、CDC2的变化，划痕实验检测干扰前后细胞的迁移能力。结果：干扰后SACC-M细胞FOXM1表达明显下调，细胞增殖受到抑制，细胞周期S期比例明显下降，CyclinB表达明显下降，但细胞的迁移能力没有明显的变化。结论：FOXM1可调节涎腺腺样囊性癌细胞的增殖，但对其迁移能力没有明显作用。

Abstract: Objective：To study the effect of FOXM1 gene on proliferation of salivary adenoid cystic carcinoma cells. Methods：The highly metastatic adenoid cystic carcinoma cell line (SACC-M) was transient transfected by constructing FOXM1 siRNA interference clips. The interference efficiency was examined by Western blotting. Cell cycle changes were determined with flow cytometry. Cell proliferation was evaluated by MTT. The protein expression of CyclinB, CDC2 was examined by Western blotting. Cell migration was examined by wound-healing assay. Results：FOXM1 siRNA transfection specifically down-regulated the FOXM1 protein in SACC-M cells. Proliferation of SACC-M cells was suppressed，and the percentage of S stage cells was significantly reduced. Cells migration did not show a clear change. Conclusions：FOXM1 could regulate cell proliferation of salivary adenoid cystic carcinoma cells, but had no obvious effects on cell migration.

中图分类号:

R739.8

丁旭李怀奇武和明吴煜农. siRNA干扰FOXM1基因对涎腺腺样囊性癌细胞增殖的影响[J]. , 2012, 3(3): 120-123.

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