生物可降解聚碳酸丁二醇酯电纺膜生物相容性的研究

›› 2012, Vol. 3 ›› Issue (3): 124-127.

生物可降解聚碳酸丁二醇酯电纺膜生物相容性的研究

姚菁¹,彭莎莎¹,陈刚²,邵成华³,沈新元³,夏阳⁴,章非敏⁴

1. 南京医科大学口腔医学研究所；南京医科大学附属口腔医院修复科
2. 南京医科大学口腔医学研究所?附属口腔医院修复科
3. 纤维材料改性国家重点实验室，东华大学材料科学与工程学院
4. 南京医科大学口腔医学研究所附属口腔医院修复科

收稿日期:2012-06-21 修回日期:2012-08-08 出版日期:2012-09-25 发布日期:2012-10-08
通讯作者: 夏阳 E-mail:xiayangxy@sina.com
基金资助:
苏州市国际科技合作项目;江苏高校优势学科建设工程资助项目;江苏省医学领军人才与创新团队

Biocompatibility of a new biodegradable poly butylene carbonate electrospun films

Received:2012-06-21 Revised:2012-08-08 Online:2012-09-25 Published:2012-10-08

摘要/Abstract

摘要： 目的：对一种新型生物可降解聚碳酸丁二醇酯（poly butylene carbonate，PBC）电纺膜的生物相容性进行研究。方法：以聚己内酯（polycaprolactone，PCL）电纺膜为对照，检测2种膜的表面接触角，然后分别在其上接种MC3T3-E1细胞，使用场发射扫描电子显微镜对电纺膜的原始形貌和接种细胞1 d后的形貌进行表征，使用甲基噻唑基四唑（MTT）比色法和碱性磷酸酶（alkaline phosphatase，ALP）活性法检测电纺膜对测试细胞增殖和分化的影响。结果：PBC电纺膜的接触角值低于PCL电纺膜，且差异显著（P<0.05）；1 d后细胞在两种电纺膜的表面都铺展良好，结合紧密；PBC电纺膜和PCL电纺膜对细胞增殖活性的影响在1 d和7 d都没有显著差异（P>0.05）；7 d时，PBC电纺膜组的ALP活性高于PCL电纺膜组，且差异显著（P<0.05）。结论：与电纺PCL膜相比，电纺PBC膜在亲水性和促进细胞分化方面性能更佳，可以进一步研发其在生物医学领域的应用。

Abstract: Objective：To study the biocompatibility of a new kind of biodegradable poly butylene carbonate (PBC) electrospun film. Methods：Polycaprolactone (PCL) electrospun membranes were prepared as control. Contact angles on the surfaces of two kinds of films were tested. Cell line MC3T3-E1 was seeded onto both kinds of membranes. SEM images were taken to characterize the original morphology of electrospun films and cells one day after seeding. MTT and ALP tests were performed to test the influence of electrospun films on proliferation and differentiation of tested cells. Results：Contact angles in PBC group were lower than those in PCL group, and the difference was significant (P<0.05). One day after seeding, cells attached tightly with both PBC and PCL membranes and spread well on them. There were no significant differences in effects on cell proliferation between two kinds of membranes on the 1st and 7th day (P>0.05), while ALP values in PBC group were significantly higher than those in PCL group on the 7th day (P<0.05). Conclusions：Compared with PCL films, PBC films were a little better in hydrophilicity and cell differentiation, and can be further studied for biomedical use.

中图分类号:

R318

姚菁彭莎莎陈刚邵成华沈新元夏阳章非敏. 生物可降解聚碳酸丁二醇酯电纺膜生物相容性的研究[J]. , 2012, 3(3): 124-127.