富血小板纤维蛋白对兔脂肪干细胞成骨分化的影响

›› 2013, Vol. 4 ›› Issue (1): 19-22.

富血小板纤维蛋白对兔脂肪干细胞成骨分化的影响

徐炜¹,王明国²,马锋²,杨世茂²,林夏莲³,于彦领³,张新新³

1. 潍坊医学院口腔学院
2. 济南市中心医院口腔科
3. 潍坊医学院

收稿日期:2013-01-02 修回日期:2013-03-08 出版日期:2013-03-25 发布日期:2013-03-30
通讯作者: 王明国 E-mail:xuwei1987010@163.com

Effects of platelet-rich fibrin on osteogenic differentiation of the rabbit adipose-derived stem cells

Received:2013-01-02 Revised:2013-03-08 Online:2013-03-25 Published:2013-03-30

摘要/Abstract

摘要： 目的：体外分离培养兔脂肪干细胞（adipose-derived stem cells，ADSCs），鉴定其分化能力并观察富血小板纤维蛋白（platelet-rich fibrin，PRF）对ADSCs成骨分化的影响。方法：取新西兰兔腹股沟处的脂肪组织，将其分离获得脂肪干细胞，培养至第三代用于实验。分别以油红O、茜素红染色鉴定其成脂和成骨分化能力。取兔耳中央动脉血一次离心法制备PRF膜。将脂肪干细胞分为2组：对照组不含PRF膜；实验组含PRF膜。用碱性磷酸酶AKP试剂盒检测不同时间点PRF对ADSCs成骨分化的影响。结果：脂肪干细胞呈长梭形贴壁生长；油红O及茜素红染色均呈阳性；在不同的时间点，实验组碱性磷酸酶活性值均高于对照组（P＜0.05)。结论：ADSCs具有成骨的潜能且PRF可以促进ADSCs向成骨细胞分化。

Abstract: Objective：To Separate and cultivate rabbit adipose-derived stem cells (ADSCs) ,identify its differentiation activity and observe the effects of Platelet-rich fibrin（PRF）on the osteogenic differentiation of thoses cells. Methods：Iguinal fat tissue extracted from New Zealand rabbits and isolated adipose derived stem cells. The third generation ADSCs were used in the experiment. The methods of oil red O and alizarin red staining were carried out to identify the dipogenic and osteogenic differentiation .Blood collection was carried out from central artery of the rabbit and obtain the PRF membrane.The ADSCs were divided into two groups: the experiment group with a PRF membrane ;the control group without a PRF membrane.The alkaline phosphatase kit was used to observe the effect of PRF on the osteogenic differentiation of ADSCs.Results:The rabbit ADSCs grew in the way of long spindle adherence.The results of oil red O and alizarin red staining of the cells were positive. At different time points, the expression of alkaline phosphatase in the experiment group was significantly higher than the control group (p < 0.05). Conclusions：ADSCs can induced osteogenic differentiation and PRF can promote ADSCs to differentiate into osteoblasts.

中图分类号:

R329.8

徐炜王明国马锋杨世茂林夏莲于彦领张新新. 富血小板纤维蛋白对兔脂肪干细胞成骨分化的影响[J]. , 2013, 4(1): 19-22.

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