牙本质非胶原蛋白对人牙髓干细胞增殖活性及矿化能力的影响

›› 2013, Vol. 4 ›› Issue (1): 15-18.

牙本质非胶原蛋白对人牙髓干细胞增殖活性及矿化能力的影响

蒋玉姣¹,²,曹灵³,俞艳²,王利娟²,于金华²,张光东²

1. 南京医科大学附属口腔医院牙体牙髓科
2. 南京医科大学口腔医学研究所
3. 南京医科大学附属口腔医院

收稿日期:2013-01-02 修回日期:2013-01-26 出版日期:2013-03-25 发布日期:2013-03-30
通讯作者: 张光东 E-mail:edg_zhang@yahoo.com.cn
基金资助:
江苏省自然科学基金

Effects of dentin non-collagenous proteins on the proliferation and mineralization of human dental pulp stem cells

Received:2013-01-02 Revised:2013-01-26 Online:2013-03-25 Published:2013-03-30

摘要/Abstract

摘要： 目的：明确牙本质非胶原蛋白(dentin non-collagenous proteins，dNCPs)对人牙髓干细胞(human dental pulp stem cells, hDPSCs)增殖及矿化能力的影响。方法：通过MTT法、碱性磷酸酶(alkalinephosphotase，ALP)活性检测、茜素红钙化结节染色和氯代十六烷基吡啶定量分析钙离子浓度，检测10 μg/mL dNCPs对hDPSCs增殖和矿化能力的影响。结果：dNCPs分别诱导1、3、5、7、9 天，人牙髓干细胞增殖活性与对照组无显著性差异(P>0.05)；诱导3、5、7天时，人牙髓干细胞ALP活性明显上调，与对照组相比有统计学差异(P<0.01)；诱导2周后，茜素红染色显示10 μg/mL dNCPs组出现较大钙化结节，定量分析显示，其形成钙化结节的能力明显高于对照组(P<0.001)。结论：10 μg/mL dNCPs可明显促进人牙髓干细胞的矿化能力，对其增殖活性的影响则不明显。

Abstract: Objective: To identify the effects of dentin non-collagenous proteins (dNCPs) on the proliferation and mineralization of human dental pulp stem cells (hDPSCs) in vitro. Methods: hDPSCs were treated with 10 μg/mL dNCPs in normal medium for certain time periods. Proliferation of hDPSCs was evaluated by MTT colorimetric assay. Alkalinephosphotase (ALP) activity was determined by enzymekinetics methods. Alizarin red staining and cetylpyridinium chloride (CPC) assay were performed to investigate the mineralization ability of hDPSCs. Results: There was no difference between the proliferation activity of hDPSCs treated with dNCPs and the other group (P>0.05). ALP activity and mineralization ability of this group were signigicantly upregulated (P<0.01). Conclusions: 10 μg/mL dNCPs significantly promotes the mineralization of hDPSCs, while the proliferation ability shows no significant difference.

中图分类号:

R329.8

蒋玉姣曹灵俞艳王利娟于金华张光东. 牙本质非胶原蛋白对人牙髓干细胞增殖活性及矿化能力的影响[J]. , 2013, 4(1): 15-18.

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