少突胶质细胞谱系基因－髓鞘碱性蛋白在口腔扁平苔藓组织中的表达

›› 2013, Vol. 4 ›› Issue (1): 11-14.

少突胶质细胞谱系基因－髓鞘碱性蛋白在口腔扁平苔藓组织中的表达

王燃,单婧,马江敏,周婷君,范媛

南京医科大学口腔医学研究所

收稿日期:2013-01-08 修回日期:2013-02-28 出版日期:2013-03-25 发布日期:2013-03-30
通讯作者: 范媛 E-mail:fanyuan65@hotmail.com
基金资助:
国家自然科学基金资助项目

Expression of genes of the oligodendrocyte lineage-myelin basic protein in oral lichen planus

Received:2013-01-08 Revised:2013-02-28 Online:2013-03-25 Published:2013-03-30

摘要/Abstract

摘要： [摘要] 目的探讨少突胶质细胞谱系基因-髓鞘碱性蛋白(genes of the oligodendrocyte lineage-myelin basic protein，Golli-MBP)在口腔扁平苔藓（oral lichen planus，OLP）组织中的表达及其与OLP发病机制的关系。方法采用实时荧光定量PCR的方法，检测39例OLP患者病损组织和16例健康口腔黏膜组织中Golli-MBP的基因表达水平，采用免疫组化法检测38例OLP及20例正常口腔粘膜组织石蜡标本中Golli-MBP的表达情况。结果 Golli-MBP mRNA在OLP组中的表达显著高于正常对照组(P<0.001)。 OLP患者石蜡标本中Golli-MBP的表达水平亦显著高于对照组（P<0.001）；固有层淋巴细胞中Golli-MBP的表达高于对照组（P=0.001）；上皮细胞中Golli-MBP的表达在OLP患者及对照组间无统计学差异（P＞0.05）。结论 Golli-MBP在OLP组织中高表达，可能在OLP的发病机制中起一定作用。

Abstract: Abstract： Objective To study the expression of Golli-MBP (genes of the oligodendrocyte lineage-myelin basic protein，Golli-MBP) in lesions of oral lichen planus (OLP). Methods The mRNA expression level of Golli-MBP in 39 OLP tissues and 16 normal oral mucosa tissues was detected by real-time fluorescence quantitative PCR. The expression of Golli-MBP was also investigated in OLP (38 cases) and normal oral mucosa specimens (20 cases) using immunohistochemical technique. Results Increased Golli-MBP mRNA expression was found in OLP than that in normal oral mucosa(P<0.001). Expression of Golli-MBP in paraffin-embedded biopsy specimens was significantly higher in OLP than in normal controls(P<0.001); Golli-MBP expressed significantly higher in lymphocytes of OLP than in normal controls (P<0.05), while there were no significant differences in epithelial cells（P＞0.05）. Conclusions The data accumulated here indicate that the overexpression of Golli-MBP in OLP may play a role in the pathogenesis of OLP.

中图分类号:

R781.5

王燃单婧马江敏周婷君范媛. 少突胶质细胞谱系基因－髓鞘碱性蛋白在口腔扁平苔藓组织中的表达[J]. , 2013, 4(1): 11-14.

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