过表达BCOR-short基因抑制根尖牙乳头干细胞成骨分化功能

›› 2018, Vol. 9 ›› Issue (1): 12-16.

• 论著 • 上一篇下一篇

过表达BCOR-short基因抑制根尖牙乳头干细胞成骨分化功能

王海锋¹,幸丹²,³,刘亚男¹,刘思思¹,宁美芝¹,李文峰¹,曹钰⁴

1. 首都医科大学康复医学院，中国康复研究中心北京博爱医院北京
2. 中国康复研究中心北京博爱医院口腔科
3. 马里兰大学牙学院生物材料及组织工程实验室
4. 首都医科大学附属北京口腔医院综合科

收稿日期:2018-01-05 修回日期:2018-02-26 出版日期:2018-03-25 发布日期:2018-04-04
通讯作者: 曹钰 E-mail:caoyu_bj@163.com
基金资助:
中国康复研究中心重点项目

Over-expression of BCOR-short inhibited the osteogenic differentiation potential of stem cells from apical papilla

Received:2018-01-05 Revised:2018-02-26 Online:2018-03-25 Published:2018-04-04
Contact: Yu ?CAO E-mail:caoyu_bj@163.com

摘要/Abstract

摘要： 目的：研究BCL6共抑制因子（BCOR）异构体（BCOR-short）对根尖牙乳头干细胞（SCAPs）成骨分化能力的影响。方法：利用HA-BCOR-short逆转录病毒载体在SCAPs中过表达BCOR-short，Western Blot检测过表达效果；通过碱性磷酸酶（ALP）活性、茜素红染色及钙离子定量分析研究SCAPs体外成骨分化能力；实时定量RT-PCR检测SCAPs中骨涎蛋白（BSP）、骨钙素（OCN）和转录因子AP2A的表达。结果：Western Blot结果显示HA-BCOR-short可以在SCAPs有效的过表达；ALP活性结果显示过表达HA-BCOR-short抑制SCAPs的ALP活性；茜素红染色及钙离子定量分析结果显示HA-BCOR-short明显抑制SCAPs体外矿化能力；实时定量RT-PCR结果显示过表达HA-BCOR-short明显抑制SCAPs中BSP、OCN和AP2A的表达。结论：过表达BCOR异构体BCOR-short可以抑制转录因子AP2A的表达，并且抑制SCAPs体外成骨分化功能。

Abstract: Objective：To investigate the effect of BCOR-short on the osteogenic differentiation potential of stem cells from apical papilla (SCAPs). Methods：Retroviral HA-BCOR-short was used to over-express the BCOR-short in SCAPs. Western Blot was used to detect the expression of HA-BCOR-short. Alkaline phosphatase (ALP) activity assay was used to detect the early marker of osteogenic differentiation-ALP activity, Alizarin-red staining and quantitative analysis of calcium were used to investigate the mineralization potential of SCAPs in vitro. Real time RT-PCR was used to detect the osteogenesis related genes expressions bone sialoprotein (BSP), osteocalcin (OCN) and AP2A. Results：Western Blot result showed that HA-BCOR-short could over-express in SCAPs. The over-expression of HA-BCOR-short inhibited ALP activity, mineralization, and the expressions of BSP and OCN in SCAPs. The AP2A expression was repressed in HA-BCOR-short over-expressed SCAPs compared with control group. Conclusions：Our results indicated that over-expression of HA-BCOR-short repressed osteogenic differentiation potential of SCAPs and the expression of AP2A.

王海锋幸丹刘亚男刘思思宁美芝李文峰曹钰. 过表达BCOR-short基因抑制根尖牙乳头干细胞成骨分化功能[J]. , 2018, 9(1): 12-16.

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