CCL25/CCR9在口腔扁平苔藓外周血的表达及对T细胞功能的影响

›› 2019, Vol. 10 ›› Issue (3): 118-122.

CCL25/CCR9在口腔扁平苔藓外周血的表达及对T细胞功能的影响

张婷婷¹,王琛²,沈晨³,朱丹丹¹,李姗¹,范媛⁴

1. 南京医科大学口腔疾病研究江苏省重点实验室；南京医科大学附属口腔医院口腔黏膜科
2. 南京医科大学附属口腔医院口腔黏膜病科
3. 南京医科大学附属口腔医院
4. 南京医科大学口腔医学研究所

收稿日期:2019-01-18 修回日期:2019-08-20 出版日期:2019-09-25 发布日期:2019-09-25
通讯作者: 范媛 E-mail:fanyuan65@hotmail.com
基金资助:
国家自然科学基金;江苏高校优势学科建设工程资助项目

Expression of CCL25/CCR9 in peripheral blood of oral lichen planus and its effect on T cell function

Received:2019-01-18 Revised:2019-08-20 Online:2019-09-25 Published:2019-09-25

摘要/Abstract

摘要： 摘要：目的：探讨CCL25/CCR9在口腔扁平苔藓（oral lichen planus，OLP）发病机制中的作用。方法：采用实时荧光定量PCR（Quantities Real-Time PCR,qPCR）、WES全自动蛋白印迹法(Simple Western assays)和酶联免疫吸附试验（ELISA）检测OLP外周血CCL25和CCR9的表达。采用Transwell、CCK8、流式细胞术检测并分析CCL25/CCR9对T细胞功能的影响。结果：OLP外周血T细胞CCR9和CCL25mRNA及CCL25蛋白表达显著降低（P＜0.01）。CCL25诱导T细胞迁移，OLP外周血T细胞趋化指数（chemotactic index，CI）1.4低于对照组1.8(P＜0.01)，anti-CCR9mAb阻断后CI显著降至0.9(P＜0.05)。OLP外周血T细胞增殖率升高、凋亡率下降，但CCL25及anti-CCR9mAb对增殖率、凋亡率并无影响。结论：CCL25和CCR9在OLP外周血中低表达，其相互作用可介导T细胞迁移。CCL25/CCR9在OLP发病过程中可能主要参与炎细胞的募集过程。

关键词: 关键词：CCL25/CCR9, 口腔扁平苔藓, T细胞, 迁移, 增殖, 凋亡

Abstract: Abstract:Objective:To explore the role of CCL25/CCR9 in pathogenesis of oral lichen planus (OLP). Methods： The expression of CCL25 in plasma and CCR9 of peripheral blood T cell of OLP were detected by qRT-PCR, WES and ELISA. Cultured T lymphocytes in vitro，the effects of CCL25/CCR9 on T cell migration, proliferation and apoptosis were detected by Transwell assay, CCK8 and flowcytometry. Results：The expression of peripheral blood T cell CCR9, CCL25 mRNA and CCL25 protein in plasma of OLP were decreased compared with those of the control group (P<0.01). CCL25 induces T cell to migrate, and the chemotactic index (CI) of OLP is lower than that of the control group (P<0.01). CI reduced significantly after blocked by anti-CCR9 mAb（P<0.05). Compared with the control group, the proliferation of T cell in peripheral blood of OLP was increased and the apoptosis was decreased, but they were not associated with the addition of CCL25 or anti-CCR9 mAb. Conclusions： CCL25 and CCR9 were lowly expressed in peripheral blood of OLP and their interaction can mediate T cell migrating. CCL25/CCR9 may be involved in recruitment of inflammatory cells during the pathogenesis of OLP.

Key words: Key words:CCL25/CCR9, OLP, T lymphocytes, migration, proliferation, apoptosis

张婷婷王琛沈晨朱丹丹李姗范媛. CCL25/CCR9在口腔扁平苔藓外周血的表达及对T细胞功能的影响[J]. , 2019, 10(3): 118-122.

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