TEAD4表达与口腔鳞癌患者不良预后及其促进细胞迁移侵袭作用研究

›› 2020, Vol. 11 ›› Issue (1): 36-40.

TEAD4表达与口腔鳞癌患者不良预后及其促进细胞迁移侵袭作用研究

李晋¹,张伟¹,李中武¹,程杰²,杨建荣¹

1. 南京医科大学附属口腔医院
2. 南京医科大学附属口腔医院口腔颌面外科

收稿日期:2019-10-23 修回日期:2020-02-25 出版日期:2020-03-25 发布日期:2020-04-07
通讯作者: 杨建荣 E-mail:m15950686940@163.com
基金资助:
TAZ/TEAD4-SOX2调控轴参与口腔鳞癌肿瘤干细胞干性维持的机制研究

Prognostic role of TEAD4 in Oral Squamous Cell Carcinoma and its Effect on cell migration and invasion

Received:2019-10-23 Revised:2020-02-25 Online:2020-03-25 Published:2020-04-07
Contact: Jian-rong -Yang E-mail:m15950686940@163.com

摘要/Abstract

摘要： 目的：研究TEA转录因子4（TEAD4）在口腔鳞癌（OSCC）组织中的表达与临床病理参数、预后的关系。方法：免疫组化检测79例原发性OSCC标本和21例口腔正常上皮黏膜中TEAD4的表达水平，分析其与临床病理参数之间的关系。使用siRNA转染沉默Cal27细胞内源性TEAD4，通过Western blot、qRT-PCR、划痕试验、Transwell侵袭等实验探究TEAD4沉默对OSCC细胞迁移、侵袭的影响。结果：免疫组化结果显示TEAD4在OSCC组织中表达明显高于正常口腔黏膜（P＜0.05）。TEAD4高表达与患者颈淋巴结转移、临床分期和患者总体生存率相关（P＜0.05）。TEAD4沉默后Cal27细胞侵袭、迁移能力明显减弱。结论：TEAD4表达与OSCC恶性表型及预后有关，参与OSCC细胞迁移、侵袭表型的调控。

关键词: TEA转录因子4, 口腔鳞癌, 迁移, 侵袭

Abstract: Objective: To investigate the expression of TEA domain transcription factor 4 (TEAD4) in oral squamous cell carcinoma (OSCC) and its correlation with clinical pathological parameters and prognosis value, and explore the biological function in OSCC invasion and migration ability. Methods: Immunohistochemistry was assessed to detect TEAD4 expression in 79 primary OSCC sample and 21 normal oral mucosa. The associations between TEAD4 and multiple clinicopathological parameters and prognosis were analyzed. In vivo assay, Western Blot, q-PCR, wound healing assay and Transwell invasion assay were assessed to detect the invasion and migration ability when TEAD4 silencing. Results: Immunohistochemistry data showed that the expression of TEAD4 in OSCC was significantly higher than that in normal oral mucosa (P＜0.05). And its expression in OSCC was significantly correlated with cervical lymph node metastasis, clinical stage, and overall survival rate (P＜0.05). The invasion and migration ability of Cal27 was impaired when TEAD4 silencing in vivo assay. Conclusions: Our findings reveal that overexpression of TEAD4 correlate with OSCC malignant phenotype of, the prognosis of patients and promoting OSCC cell migration and invasion.

Key words: TEAD4, oral squamous cell carcinoma, cell migration, invasion

中图分类号:

R782

李晋张伟李中武程杰杨建荣. TEAD4表达与口腔鳞癌患者不良预后及其促进细胞迁移侵袭作用研究[J]. 口腔生物医学, 2020, 11(1): 36-40.

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