基于IL-1β的纳米金免疫层析试纸法在牙周炎GCF检测中的应用基础研究

›› 2020, Vol. 11 ›› Issue (1): 45-48.

基于IL-1β的纳米金免疫层析试纸法在牙周炎GCF检测中的应用基础研究

滕蕊,贺望虹,许莹,孙帼,苟建重,李昂

西安交通大学口腔医院

收稿日期:2019-12-31 修回日期:2020-02-08 出版日期:2020-03-25 发布日期:2020-04-07
通讯作者: 李昂 E-mail:drliang@mail.xjtu.edu.cn

A basic study on the application of gold immunochromatographic assay based on il-1 in GCF detection of periodontitis

Received:2019-12-31 Revised:2020-02-08 Online:2020-03-25 Published:2020-04-07

摘要/Abstract

摘要： 目的：研发基于胶体金的免疫层析试纸，用于龈沟液（GCF）中IL-1β水平的即时检测，为牙周炎病变的精确诊断提供实验依据。方法：首先使用柠檬酸三钠还原氯金酸法制备胶体金，优化反应条件后制备胶体金-抗体复合物，组装胶体金层析试纸后测定其标准曲线，检测临床样本，并与酶联免疫吸附试验（ELISA）检测结果做Spearman相关分析。结果：成功制备了胶体金免疫层析试纸，检测灵敏度可达到5 ng/mL，临床样本检测显示，胶体金试纸与ELISA检测慢性牙周炎组龈沟液IL-1β水平结果的Spearman相关系数为0.992，相关性良好。结论：本研究研发的胶体金免疫层析试纸检测结果具有可靠性，对未来牙周炎患者GCF中IL-1β水平的体外快速检测提供了实验基础。

关键词: 牙周炎, IL-1β, 即时检测, 纳米金, 免疫层析试纸

Abstract: Objective: To develop a colloidal gold-based immunochromatographic test strip for the rapid detection of IL-1β levels in the gingival crevicular fluid (GCF), expecting to provide experimental evidence for the accurate diagnosis of chronic periodontitis. Methods: The colloidal gold was prepared by chloroauric acid and trisodium citrate. After optimizing the reaction conditions, the colloidal gold-antibody complex was prepared. Then, with the assembled colloidal gold chromatography test strips, the standard curve was tested and the clinical samples were detected, whose results were analyzed by Spearman correlation with those of ELISA. Results: The colloidal gold immunochromatographic test strips were successfully prepared, and the detection sensitivity could reach 5 ng/mL. The clinical samples detection showed that the Spearman correlation coefficient between the test strips and ELISA was 0.992 for detecting IL-1β levels in GCF in periodontitis patients. Conclusion: The developed colloidal gold-based immunochromatographic test strips can perform reliable detections, which provide experimental evidence for rapid detection of IL-1β levels in GCF in periodontitis patients.

Key words: IL-1β, Point-of-Care Testing, GNPs, lateral flow strips.

滕蕊贺望虹许莹孙帼苟建重李昂. 基于IL-1β的纳米金免疫层析试纸法在牙周炎GCF检测中的应用基础研究[J]. 口腔生物医学, 2020, 11(1): 45-48.

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