牙龈卟啉单胞菌诱导的耐受对小鼠牙槽骨吸收的影响

›› 2020, Vol. 11 ›› Issue (1): 28-31.

牙龈卟啉单胞菌诱导的耐受对小鼠牙槽骨吸收的影响

朱奕名,方媛春,汪悦,张尧尧,孙颖

南京医科大学口腔医学院

收稿日期:2019-10-23 修回日期:2019-11-11 出版日期:2020-03-25 发布日期:2020-04-07
通讯作者: 孙颖 E-mail:ebolasun@163.com
基金资助:
国家自然科学基金;江苏省自然科学基金;江苏省高校优势学科建设工程资助项目

Tolerance Induced by Porphyromonas gingivalis Alleviates Periodontal Alveolar Bone Resorption in Mice

Received:2019-10-23 Revised:2019-11-11 Online:2020-03-25 Published:2020-04-07

摘要/Abstract

摘要： 目的：研究牙龈卟啉单胞菌（P.gingivalis）诱导的耐受对小鼠骨吸收相关因子IL-1β、骨保护素（OPG）/NF-κB受体激活蛋白配体（RANKL）表达水平，以及牙槽骨吸收的影响。方法：采用1×109 CFU P.gingivalis口内涂布给菌5 d，磷酸盐缓冲液（PBS）洗脱后，再次口内涂布P.gingivalis给菌2 d，构建Balb/c小鼠牙周组织耐受模型。10 d后收集新鲜牙龈组织，采用western bolt检测牙龈组织中IL-1β、OPG和RANKL表达水平的变化。6周后，收集上颌骨，采用亚甲基蓝染色法检测牙槽骨吸收水平的改变。结果：耐受组小鼠牙龈组织中IL-1β表达水平较非耐受组降低，OPG/RANKL比值较非耐受组升高。亚甲基蓝染色结果显示，耐受组小鼠釉牙骨质界至牙槽嵴顶（ABC-CEJ）的距离小于非耐受组（P＜0.01），说明耐受组小鼠牙槽骨吸收较非耐受组降低。结论：P.gingivalis诱导小鼠牙周组织耐受后，小鼠牙龈组织中骨吸收相关因子IL-1β分泌降低，OPG/RANKL比值升高，抑制了牙槽骨吸收。

关键词: 牙龈卟啉单胞菌, 耐受, 牙槽骨, 吸收

Abstract: Objective: To investigate the effects of tolerance induced by Porphyromonas gingivalis (P.gingivalis) on bone resorption related cytokines, including IL-1β, osteoprotegerin (OPG)/receptor activator of NF-κB ligand (RANKL), and alveolar bone resorption in mice. Methods: Tolerance in murine periodontal tissues was induced by orally inoculation with 1×109CFU P.gingivalis for 5 days, washing with PBS and inoculation with P.gingivalis again for additional 2 days. After 10 days, fresh gingivae were collected and the expression levels of IL-1β, OPG and RANKL were detected by western bolt. 6 weeks later, maxillae were collected and the distances from the cementoenamel junction (CEJ) to the alveolar bone crest (ABC) were evaluated by methylene blue staining. Results: The expression levels of IL-1β in gingivae from P.gingivalis-tolerant mice were lower than those from non-tolerant group and the ratio of OPG/RANKL expression levels was higher than that of non-tolerant group. Less bone absorption was observed in tolerant group compared with that in non-tolerant mice (P<0.05). Conclusion:Tolerance induced by P.gingivalis could suppress

Key words: Porphyromonas gingivalis, tolerance, alveolar bone, resorption

朱奕名方媛春汪悦张尧尧孙颖. 牙龈卟啉单胞菌诱导的耐受对小鼠牙槽骨吸收的影响[J]. 口腔生物医学, 2020, 11(1): 28-31.

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