基于液相色谱-质谱技术的口腔癌代谢组学初步研究

doi:10.3969/j.issn.1674-8603.2020.02.009

›› 2020, Vol. 11 ›› Issue (2): 107-112.doi: 10.3969/j.issn.1674-8603.2020.02.009

基于液相色谱-质谱技术的口腔癌代谢组学初步研究

毛广艳¹,孙秋望月²,邓威²,金齐尧³,王晨星⁴,李怀奇³,叶金海¹

1. 南京医科大学附属口腔医院颌面外科
2. 南京医科大学附属口腔医院口腔颌面外科
3. 南京医科大学附属口腔医院
4. 1.南京医科大学口腔疾病研究江苏省重点实验室 2.南京医科大学附属口腔医院口腔颌面外科

收稿日期:2020-02-25 修回日期:2020-05-06 出版日期:2020-06-25 发布日期:2020-07-08
通讯作者: 毛广艳 E-mail:2249168634@qq.com
基金资助:
国家自然基金;“科教强卫工程”医学重点人才项目;江苏高校优势学科建设工程资助项目

A preliminary study on the metabonomics of oral cancer based on liquid chromatography-mass spectrometry

Received:2020-02-25 Revised:2020-05-06 Online:2020-06-25 Published:2020-07-08

摘要/Abstract

摘要： 目的：采用液相色谱-质谱联用技术，探讨口腔鳞癌（OSCC）组织与匹配的癌旁组织在代谢组学方面的差异。方法：基于液相色谱-质谱联用技术对15对OSCC组织及匹配的癌旁组织进行非靶向代谢组学分析。采用主成分分析及偏最小二乘法判别分析方法，筛选并确定差异代谢物。用MetaboAnalyst数据库对代谢物进行代谢集富集分析及代谢通路拓扑分析，确定异常代谢通路。结果：对OSCC组织及匹配的癌旁组织进行分析得到16个差异代谢物；经过代谢通路分析，OSCC患者体内可能存在鞘脂代谢、精氨酸和脯氨酸代谢、嘧啶代谢和甘油磷脂代谢等代谢通路异常。结论：OSCC组织跟匹配的癌旁组织在代谢组学上存在明显代谢异常，具有诊断价值的异常代谢物可作为OSCC早期诊断标志物。

关键词: 口腔鳞癌, 代谢组学, 超高效液相色谱-质谱, 差异代谢物

Abstract: Objective:?To determine the metabolic differences between oral squamous cell carcinoma (OSCC) tissue and the paired adjacent noncancerous tissue by liquid chromatography-mass spectrometry. Methods:Fifteen pairs of OSCC tissues and matched normal tissues were analyzed by liquid chromatography-mass spectrometry. The differential metabolites were screened and determined by principal component analysis and partial least squares discriminant analysis.?Using MetaboAnalyst database to do the metabolic set enrichment analysis and metabolic pathway topological analysis of metabolites for determining abnormal metabolic pathways. Results:? Sixteen different metabolites can we got in OSCC tissues and adjacent tissues after analysis. By metabolic pathway analysis, there may be abnormal metabolic pathways such as sphingolipid metabolism, arginine and proline metabolism, pyrimidine metabolism and glycerophospholipid metabolism in OSCC patients. Conclusions:?There are obvious metabolic abnormalities in the tissues of OSCC and the paired adjacent noncancerous tissue, and abnormal metabolites with diagnostic value can be used as early diagnostic markers for OSCC patients.

Key words: oral squamous cell carcinoma, Metabolomics, UPLC-MS/MS, Differential metabolites

毛广艳孙秋望月邓威金齐尧王晨星李怀奇叶金海. 基于液相色谱-质谱技术的口腔癌代谢组学初步研究[J]. 口腔生物医学, 2020, 11(2): 107-112.

参考文献 26

[1]	Du M, Nair R, Jamieson L, et al.Incidence Trends of Lip, Oral Cavity, and Pharyngeal Cancers: Global Burden of Disease 1990-2017[J].J Dent Res, 2020, 99(2):143-151
[2]	Ghani WMN, Ramanathan A, Prime SS, et al.Survival of Oral Cancer Patients in Different Ethnicities[J].Cancer Invest, 2019, 37(7):275-287
[3]	Ishikawa S, Sugimoto M, Edamatsu K, et al.Discrimination of oral squamous cell carcinoma from oral lichen planus by salivary metabolomics[J].Oral Dis, 2020, 26(1):35-42
[4]	Sridharan G, Ramani P, Patankar S, et al.Evaluation of salivary metabolomics in oral leukoplakia and oral squamous cell carcinoma[J].J Oral Pathol Med, 2019, 48(4):299-306
[5]	Mikkonen JJW, Singh SP, Akhi R, et al.Potential role of nuclear magnetic resonance spectroscopy to identify salivary metabolite alterations in patients with head and neck cancer[J].Oncol Lett, 2018, 16(5):6795-6800
[6]	Lohavanichbutr P, Zhang Y, Wang P, et al.Salivary metabolite profiling distinguishes patients with oral cavity squamous cell carcinoma from normal controls[J].PLoS One, 2018, 13(9):e204249-
[7]	Li XZ, Zhang SN, Yang XY.Serum-based metabolomics characterization of patients with reticular oral lichen planus[J].Arch Oral Biol, 2019, 99:183-189
[8]	Hsu CW, Chen YT, Hsieh YJ, et al.Integrated analyses utilizing metabolomics and transcriptomics reveal perturbation of the polyamine pathway in oral cavity squamous cell carcinoma[J].Anal Chim Acta, 2019, 1050:113-122
[9]	Musharraf SG, Shahid N, Naqvi SMA, et al.Metabolite Profiling of Preneoplastic and Neoplastic Lesions of Oral Cavity Tissue Samples Revealed a Biomarker Pattern[J].Sci Rep, 2016, 6:38985-
[10]	Scherer HU, Huizinga TWJ, Kr?nke G, et al.The B cell response to citrullinated antigens in the development of rheumatoid arthritis[J].Nat Rev Rheumatol, 2018, 14(3):157-169
[11]	Barza? JA, Szczylik C, Rzepecki P, et al.Plasma citrulline level as a biomarker for cancer therapy-induced small bowel mucosal damage[J].Acta Biochim Pol, 2014, 61(4):615-631
[12]	Karna E, Szoka L, Huynh TYL, et al.Proline-dependent regulation of collagen metabolism[J].Cell Mol Life Sci, 2019, :-
[13]	Surazynski A, Donald SP, Cooper SK, et al.Extracellular matrix and HIF-1 signaling: the role of prolidase[J].Int J Cancer, 2008, 122(6):1435-1440
[14]	Fultang L, Vardon A, De Santo C, et al.Molecular basis and current strategies of therapeutic arginine depletion for cancer[J].Int J Cancer, 2016, 139(3):501-509
[15]	Zou S, Wang X, Liu P, et al.Arginine metabolism and deprivation in cancer therapy[J].Biomed Pharmacother, 2019, 118:109210-
[16]	Stefanescu H, Suciu A, Romanciuc F, et al.Lyso-phosphatidylcholine: A potential metabolomic biomarker for alcoholic liver disease?[J].Hepatology, 2016, 64(2):678-679
[17]	Huang X, Zeng J, Zhou L, et al.A New Strategy for Analyzing Time-Series Data Using Dynamic Networks: Identifying Prospective Biomarkers of Hepatocellular Carcinoma[J].Sci Rep, 2016, 6:32448-
[18]	Taylor LA, Arends J, Hodina AK, et al.Plasma lyso-phosphatidylcholine concentration is decreased in cancer patients with weight loss and activated inflammatory status[J].Lipids Health Dis, 2007, 6:17-
[19]	Hu Q, Wang M, Cho MS, et al.Lipid profile of platelets and platelet-derived microparticles in ovarian cancer[J].BBA Clin, 2016, 6:76-81
[20]	Joo EJ, Weyers A, Li G, et al.Carbohydrate-containing molecules as potential biomarkers in colon cancer[J].OMICS, 2014, 18(4):231-241
[21]	Indellicato R, Trinchera M.The Link between Gaucher Disease and Parkinson's Disease Sheds Light on Old and Novel Disorders of Sphingolipid Metabolism[J].Int J Mol Sci, 2019, 20(13):E3304-
[22]	Czubowicz K, J??ko H, Wencel P, et al.The Role of Ceramide and Sphingosine-1-Phosphate in Alzheimer's Disease and Other Neurodegenerative Disorders[J].Mol Neurobiol, 2019, 56(8):5436-5455
[23]	Simon J, Ouro A, Ala-Ibanibo L, et al.Sphingolipids in Non-Alcoholic Fatty Liver Disease and Hepatocellular Carcinoma: Ceramide Turnover[J].Int J Mol Sci, 2019, 21(1):E40-
[24]	Zheng X, Li W, Ren L, et al.The sphingosine kinase-1/sphingosine-1-phosphate axis in cancer: Potential target for anticancer therapy[J].Pharmacol Ther, 2019, 195:85-99
[25]	Hannun YA, Obeid LM.Sphingolipids and their metabolism in physiology and disease[J].Nat Rev Mol Cell Biol, 2018, 19(3):175-191
[26]	Kato K, Shimasaki M, Kato T, et al.Expression of Sphingosine Kinase-1 Is Associated with Invasiveness and Poor Prognosis of Oral Squamous Cell Carcinoma[J].Anticancer Res, 2018, 38(3):1361-1368

基于液相色谱-质谱技术的口腔癌代谢组学初步研究

A preliminary study on the metabonomics of oral cancer based on liquid chromatography-mass spectrometry

可视化

摘要/Abstract

引用本文

使用本文

参考文献 26

相关文章 0

编辑推荐

Metrics

本文评价