关键词: 3D打印, 乳液模板, PLGA, β-磷酸三钙, 组织工程

Abstract: Objective：3D printing technology combined with emulsion template to manufacture PLGA/β-tricalcium phosphate(β- TCP)composite scaffolds(PT)with different proportions, to screen out the most significant group, and to evaluate its potential to repair large bone defects in maxillofacial region. Methods：PLGA was dissolved in dichloromethane and made into water-in-oil emulsion. β-TCP was added in proportion and mixed evenly to form scaffolds by 3D printing. The scaffolds were recorded as PT30, PT50, PT70 according to the content of TCP. After that, a series of structural characterization, physical properties and in vitro biocompatibility tests were carried out on the scaffolds. Results：With the increase of TCP content, the surface roughness of the scaffold increased, the pore size and porosity were not affected (P > 0.05), the water absorption rate increased significantly (P < 0.001), and the mechanical properties were not significantly different (P > 0.05). Only the weight and pH of PT30 scaffold decreased most significantly (P < 0.05) during degradation, and there was no significant difference between the other two groups (P > 0.05). PT30 and PT50 scaffolds have no cytotoxicity, while PT70 scaffolds are screened out because of their cytotoxicity. In addition, the number of cell adhesion increased with the increase of TCP content, and PT30 scaffold was also screened out. Conclusions：PT50 scaffold had good water absorption and mechanical properties, and its degradation speed is suitable for new bone growth, it had no cytotoxicity and was suitable for cell growth and adhesion.

Key words: 3D printing, emulsion template, PLGA , β-tricalcium phosphate , tissue engineering