牙龈卟啉单胞菌和伴放线聚集杆菌在江苏汉族牙周炎人群中的分布研究

口腔生物医学 ›› 2021, Vol. 12 ›› Issue (2): 100-105.

牙龈卟啉单胞菌和伴放线聚集杆菌在江苏汉族牙周炎人群中的分布研究

杨勰¹,付子波²,曹羡梓²,陈佳璐²,孙颖²

1. 南京医科大学附属口腔医院
2. 南京医科大学口腔医学院

收稿日期:2021-03-22 修回日期:2021-05-17 出版日期:2021-06-25 发布日期:2021-06-25
通讯作者: 孙颖 E-mail:ebolasun@163.com
基金资助:
国家自然科学基金;江苏省高校优势学科建设工程资助项目

Prevalence and distribution of Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans in Jiangsu Han nationality periodontitis patients

Received:2021-03-22 Revised:2021-05-17 Online:2021-06-25 Published:2021-06-25

摘要/Abstract

摘要： 目的：从龈下菌斑总菌数、牙龈卟啉单胞菌（P. gingivalis）、伴放线聚集杆菌（A. actinomycetemcomitans）及白细胞毒素（leukotoxin，LTX）在江苏汉族牙周炎人群中分布的角度，比较牙周病2018年新分类与1999年分类。方法：纳入73名牙周炎患者和26名牙周健康者，分别按照牙周病2018年新分类与1999年分类对牙周炎人群分组。初诊时记录全口探诊深度（PD）、临床附着丧失（CAL）、牙龈指数（GI）和菌斑指数（PLI），并收集龈下菌斑。采用实时定量PCR检测龈下菌斑中总菌、P. gingivalis和A. actinomycetemcomitans数量，采用PCR检测A. actinomycetemcomitans毒力因子LTX的携带率，比较上述指标在不同的牙周炎分类法间的分布差异。结果：慢性牙周炎和侵袭性牙周炎患者之间，总菌和P. gingivalis数量无显著差异（P＞0.05），A. actinomycetemcomitans数量和LTX阳性率存在差异（P＜0.05），所有检出的LTX均为低毒型。不同牙周炎分期分级组间，菌量和LTX阳性率存在差异（P＜0.05）。PD、CAL、GI与总菌、P. gingivalis、A. actinomycetemcomitans数量间存在相关性（P＜0.05）。结论：2018年牙周病新分类能较好的反映江苏汉族牙周炎患者的微生物学特征。

关键词: 牙龈卟啉单胞菌, 伴放线聚集杆菌, 白细胞毒素, 牙周炎, 分类

Abstract: Objective:?To investigate the prevalence and distribution of total bacteria, Porphyromonas gingivalis (P. gingivalis), Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) and leukotoxin (LTX) in Jiangsu Han nationality periodontitits subjects categorized by classifications in 2018 and 1999. Methods:?73 patients with periodontitis and 26 periodontal healthy individuals were recruited. Periodontal clinical parameters were recorded, including probing depth (PD), clinical attachment loss (CAL), gingival index (GI) and plaque index (PLI). Levels of total bacteria, P. gingivalis and A. actinomycetemcomitans in subgingival plaque were explored by real-time PCR, and LTX was detected by conventional PCR. Results:?There were no differences in the levels of total bacteria and P. gingivalis between chronic and aggressive periodontitis (P>0.05), while differences were found when subjects were grouped by staging and grading (P＜0.05). Moreover, differences in the levels of A. actinomycetemcomitans and positive rates of LTX between chronic and aggressive periodontitis patients, between Stage Ⅰ-Ⅱ and Ⅲ-Ⅳ patients, and between Grade A and C patients were disclosed (P>0.05). All LTXs detected in this study were subtypes of low virulence. Positive correlations were confirmed between periodontal clinical parameters (PD, CAL and GI) and the levels of total bacteria, P. gingivalis and A. actinomycetemcomitans. (P＜0.05) Conclusions:?Classification for periodontal diseases in 2018 well reflected the microbiological characteristics of Jiangsu Han nationality periodontitis patients.

Key words: Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, leukotoxin, periodontitis, classifications

杨勰付子波曹羡梓陈佳璐孙颖. 牙龈卟啉单胞菌和伴放线聚集杆菌在江苏汉族牙周炎人群中的分布研究[J]. 口腔生物医学, 2021, 12(2): 100-105.

参考文献 19

[1]	Belibasakis GN, Maula T, Bao K, Lindholm M, Bostanci N, Oscarsson J, et al. Virulence and Pathogenicity Properties of Aggregatibacter actinomycetemcomitans. Pathogens. 2019;8:222.
[2]	Imbronito AV, Okuda OS, Maria de Freitas N, Moreira Lotufo RF, Nunes FD. Detection of herpesviruses and periodontal pathogens in subgingival plaque of patients with chronic periodontitis, generalized aggressive periodontitis, or gingivitis. J Periodontol. 2008;79:2313-2321.
[3]	Johansson A, Sandstr?m G, Claesson R, H?nstr?m L, Kalfas S. Anaerobic neutrophil-dependent killing of Actinobacillus actinomycetemcomitans in relation to the bacterial leukotoxicity. Eur J Oral Sci. 2000;108:136-146.
[4]	Papapanou PN, Sanz M, Buduneli N, Dietrich T, Feres M, Fine DH, et al. Periodontitis: Consensus report of workgroup 2 of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions. J Periodontol. 2018;89 Suppl 1:S173-S182.
[5]	Caton JG, Armitage G, Berglundh T, Chapple ILC, Jepsen S, Kornman KS, et al. A new classification scheme for periodontal and peri-implant diseases and conditions - Introduction and key changes from the 1999 classification. J Clin Periodontol. 2018;45 Suppl 20:S1-S8.
[6]	Lang NP, Bartold PM. Periodontal health. J Periodontol. 2018;89 Suppl 1:S9-S16.
[7]	Poulsen K, Ennibi OK, Haubek D. Improved PCR for detection of the highly leukotoxic JP2 clone of Actinobacillus actinomycetemcomitans in subgingival plaque samples. J Clin Microbiol. 2003;41:4829-4832.
[8]	Hwang AM, Stoupel J, Celenti R, Demmer RT, Papapanou PN. Serum antibody responses to periodontal microbiota in chronic and aggressive periodontitis: a postulate revisited. J Periodontol. 2014;85:592-600.
[9]	Mitchell C, Gao L, Demuth DR. Positive and negative cis-acting regulatory sequences control expression of leukotoxin in Actinobacillus actinomycetemcomitans 652. Infect Immun. 2003;71:5640-5649.
[10]	Mínguez M, Ennibi OK, Pousa X, Lakhdar L, Abdellaoui L, Sánchez M, et al. Characterization of A. actinomycetemcomitans strains in subgingival samples from periodontitis subjects in Morocco. Clin Oral Investig. 2016;20:1809-1818.
[11]	Burgess D, Huang H, Harrison P, Aukhil I, Shaddox L. Aggregatibacter actinomycetemcomitans in African Americans with Localized Aggressive Periodontitis. JDR Clin Trans Res. 2017;2:249-257.
[12]	Jensen AB, Ennibi OK, Ismaili Z, Poulsen K, Haubek D. The JP2 genotype of Aggregatibacter actinomycetemcomitans and marginal periodontitis in the mixed dentition. J Clin Periodontol. 2016;43:19-25.
[13]	Wu YM, Yan J, Chen LL, Gu ZY. Association between infection of different strains of Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans in subgingival plaque and clinical parameters in chronic periodontitis. J Zhejiang Univ Sci B. 2007;8:121-131.
[14]	Brogan JM, Lally ET, Poulsen K, Kilian M, Demuth DR. Regulation of Actinobacillus actinomycetemcomitans leukotoxin expression: analysis of the promoter regions of leukotoxic and minimally leukotoxic strains. Infect Immun. 1994;62:501-508.
[15]	Haubek D, Johansson A. Pathogenicity of the highly leukotoxic JP2 clone of Aggregatibacter actinomycetemcomitans and its geographic dissemination and role in aggressive periodontitis. J Oral Microbiol. 2014;6.
[16]	Tan KS, Woo CH, Ong G, Song KP. Prevalence of Actinobacillus actinomycetemcomitans in an ethnic adult Chinese population. J Clin Periodontol. 2001;28:886-890.
[17]	Graetz C, Mann L, Krois J, S?lzer S, Kahl M, Springer C, et al. Comparison of periodontitis patients' classification in the 2018 versus 1999 classification. J Clin Periodontol. 2019;46:908-917.
[18]	Gon?alves PF, Klepac-Ceraj V, Huang H, Paster BJ, Aukhil I, Wallet SM, et al. Correlation of Aggregatibacter actinomycetemcomitans detection with clinical/immunoinflammatory profile of localized aggressive periodontitis using a 16S rRNA microarray method: a cross-sectional study. PLoS One. 2013:e85066.
[19]	Shaddox LM, Huang H, Lin T, Hou W, Harrison PL, Aukhil I, et al. Microbiological characterization in children with aggressive periodontitis. J Dent Res. 2012;91:927-933.