EGCG和EGCG-3Me对根管牙本质粘接耐久性的研究

口腔生物医学 ›› 2021, Vol. 12 ›› Issue (4): 230-235.

EGCG和EGCG-3Me对根管牙本质粘接耐久性的研究

薛亮¹,余昊翰²,李玲³

1. 西安电子科技大学医院口腔科
2. 口腔疾病国家临床医学研究中心，军事口腔医学国家重点实验室，陕西省口腔医学重点实验室，空军军医大学口腔医院修复科，陕西西安710032
3. 西安碑林德雅口腔门诊部

收稿日期:2021-03-19 修回日期:2021-12-14 出版日期:2021-12-25 发布日期:2022-01-04
通讯作者: 李玲 E-mail:liling1922003@163.com

Effects of epigallocatechin-3-gallate and epigallocatechin-3-O-(3-O-methyl)-gallate containing functional preconditioning liquid on the boding stability of intraradicular dentin

Received:2021-03-19 Revised:2021-12-14 Online:2021-12-25 Published:2022-01-04
Contact: Ling Li E-mail:liling1922003@163.com

摘要/Abstract

摘要： 目的：评价表没食子儿茶素没食子酸酯（EGCG）和甲基化表没食子儿茶素没食子酸酯（EGCG-3Me）功能性根管预处理液对根管牙本质粘接界面稳定性的作用，为提高根管牙本质粘接界面耐久性提供策略。方法：制备浓度分别为200、400和600 μg/mLEGCG和EGCG-3Me的根管预处理液，以无菌去离子水为对照组。构建根管感染模型，激光共聚焦显微镜观察预处理液对根管内常见感染菌粪肠球菌的抗菌性能。收集84颗离体牙，桩道预备及酸蚀处理后，使用各组预处理液冲洗，制备纤维桩粘接试件，进行即刻和老化后微推出实验测试。结果：根管预处理液可对牙本质小管内定植的粪肠球菌起到杀菌作用，EGCG-3Me较EGCG杀菌作用强，且具有浓度依赖性（P＜0.05）。处理后纤维桩即刻粘接强度各组间无显著性差异（P＞0.05），但其老化后各预处理液组的粘接强度显著高于空白对照组（P＜0.05），老化后预处理液组的粘接强度较老化前无显著性差异（P＞0.05）。结论：EGCG和EGCG-3Me功能性根管预处理液对根管牙本质小管内细菌具有灭菌作用，可提高根管牙本质粘接界面的耐久性。

关键词: 表没食子儿茶素没食子酸酯, 甲基化表没食子儿茶素没食子酸酯, 抗菌, 根管牙本质, 粘接

Abstract: Objective: To evaluate the effect of epigallocatechin-3-gallate and epigallocatechin-3-O-(3-O-methyl)-gallate containing functional preconditioning liquid on the boding stability of intraradicular dentin, hoping to find a new strategy to improve the stability of intraradicular dentin bonding interface. Methods: EGCG and EGCG-3Me was incorporated into distilled water to acquire the preconditioning liquid with concentration of 200、400 and 600 μg/mL, distilled water was used as Control Group. Build root canal-infection model and observe the antibacterial effect of preconditioning liquid with scanning electron microscope (SEM) and laser scanning confocal microscope (LSCM). After post preparation, acid etching and washing with each group of preconditioning liquid, 84 teeth collected were prepared as fiber post bonding specimens.The push-out bond strength of instant testing and aging with thermocycling for 5,000 times were also tested. Results: The preconditioning liquid showed inhibiting effect on the colonized bacteria in dentinal tubule and performed better with higher concentration. The immediate push-out bond strength showed no significant difference between control group and preconditioning liquid group (P > 0.05). The aging push-out bond strength showed no obvious decrease for preconditioning liquid group (P > 0.05). Conclusions: EGCG and EGCG-3Me preconditioning liquid showed anti-bacterial effect to the colonized bacterial in dentinal tubule and enhanced the bonding stability of intraradicular dentin-adhesive interfaces.

Key words: epigallocatechin-3-gallate, epigallocatechin-3-O-(3-O-methyl)- gallate, anti-bacterial, intraradicular dentin, bonding

薛亮余昊翰李玲. EGCG和EGCG-3Me对根管牙本质粘接耐久性的研究[J]. 口腔生物医学, 2021, 12(4): 230-235.

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