Abstract: Objective:To explore whether bone-marrow mesenchymalstem cell (BMSC) could affect ameloblastoma development and recurrence by promoting the proliferation, migration, invasion of mesenchymal cell-like ameloblastoma cell (M-AMC) via up-regulating their autophagy level. Methods:?M-AMC was isolated and identified through tissue pieces and enzymatic digestion culture. The source of M-AMC was identified by Western Blot. In vitro cell cloning experiments and Flow experiments were also used to determine the expression level of M-AMC self-renewal ability. The migration and invasion ability of M-AMC at 24, 48 and 72h were determined by transwell and laying matrigel matrix gel experiments, and the invasion levels of M-AMC and autophagy were measured by Western Blot. Results: In the M-AMC obtained by primary isolation cultures, the interstitial-derived M-AMC accounts for the main population, and has certain stemness and self-renewal capacity. The number of cells proliferating from co-culture group was significantly higher than control group on day 3 and day 7 (P<0.01). The number of M-AMC migrated and invaded at the three time points of 24,48 and 72 h significantly higher than that of the control group (P<0.01). Invasion ability and autophagy level of M-AMC expression were significantly enhanced after coculture with BMSC (P<0.01). Conclusions:?The paracrine effect of BMSC could promote the proliferation, migration and invasion of M-AMC, and these phenotypes were closely related to the up-regulated autophagy in M-AMC.