伴放线聚集杆菌与人牙龈上皮细胞相互作用的研究进展

摘要/Abstract

摘要： 伴放线聚集杆菌是一种革兰氏阴性兼性厌氧菌，是牙周炎的主要致病菌之一，其具有多种毒力因子，不仅能损害牙龈上皮细胞屏障结构，逃避宿主的免疫防御，还能向深层组织入侵，破坏牙体软组织和牙槽骨。而伴放线聚集杆菌与人牙龈上皮细胞的相互作用是细菌入侵牙周组织的第一步，也是菌体引起机体免疫反应的关键。本文就伴放线聚集杆菌在人牙龈上皮细胞上的黏附与定植和与牙龈上皮细胞的相互作用两方面的研究作一综述。

关键词: 伴放线聚集杆菌, 牙龈上皮细胞, 牙周炎

Abstract: Actinobacillus actinomycetemcomitans is a gram-negative facultative anaerobe, which is one of the main pathogenic bacteria of periodontitis. With a variety of virulence factors, it can not only damage the barrier structure of gingival epithelial cells and evade the immune defense of the host, but also invade deep tissues and destroy the soft tissue and alveolar bone. The interaction between Actinobacillus actinomycetemcomitans and human gingival epithelial cells is the first step of bacterial invasion of periodontal tissue and the key to the immune response caused by the bacteria. In this paper, we review the studies on adhesion and colonization of human gingival epithelial cells by Actinobacillus actinomycetemcomitans and its interaction with gingival epithelial cells.

Key words: Actinobacillus actinomycetemcomitans, Gingival epithelial cells, Periodontitis

马晓琳于烁曲珊琳王瑞. 伴放线聚集杆菌与人牙龈上皮细胞相互作用的研究进展[J]. 口腔生物医学, 2023, (3): 192-196.

参考文献

[1] Kononen E, Gursoy M, Gursoy U K. Periodontitis: A Multifaceted Disease of Tooth-Supporting Tissues [J]. J Clin Med, 2019, 8(8): 1135.
[2] Gholizadeh P, Pormohammad A, Eslami H, et al. Oral pathogenesis of Aggregatibacter actinomycetemcomitans [J]. Microb Pathog, 2017, 113: 303-311.
[3] Oscarsson J, Claesson R, Lindholm M, et al. Tools of Aggregatibacter actinomycetemcomitans to Evade the Host Response [J]. J Clin Med, 2019, 8(7): 1079.
[4] Groeger S, Meyle J. Oral Mucosal Epithelial Cells[J]. Front Immunol, 2019, 10: 208.
[5] Fine DH, Patil AG, Velusamy SK. Aggregatibacter actinomycetemcomitans (Aa) Under the Radar: Myths and Misunderstandings of Aa and in Aggressive Periodontitis [J]. Front Immunol, 2019, 10: 728.
[6] Novak EA. Characterization of the AI-2-dependent quorum sensing pathway in Aggregatibacter actinomycetemcomitans [D]. University of louisville, 2017.
[7] Schreiner HC, Sinatra K, Kaplan JB, et al. Tight-adherence genes of Actinobacillus actinomycetemcomitans are required for virulence in a rat model [J]. Pro Natl Acad Sci U S A, 2003, 100(12): 7295-300.
[8] Danforth DR, Tang-Siegel G, Ruiz T, et al. A Nonfimbrial Adhesin of Aggregatibacter actinomycetemcomitans Mediates Biofilm Biogenesis [J]. Infect Immun, 2018, 87(1): e00704-18.
[9] Danforth DR, Melloni M, Tristano J, et al. Contribution of adhesion proteins to Aggregatibacter actinomycetemcomitans biofilm formation [J]. Mol Oral Microbiol, 2021, 36(4): 243-253.
[10] Velusamy SK, Sampathkumar V, Ramasubbu N, et al. Aggregatibacter actinomycetemcomitans colonization and persistence in a primate model [J]. Proc Natl Acad Sci U S A, 2019, 116(44): 22307-22313.
[11] Wang SS, Tang YL, Pang X, et al. The maintenance of an oral epithelial barrier [J]. Life Sci, 2019, 227: 129-136.
[12] Beklen A, Torittu A, Ihalin R, et al. Aggregatibacter actinomycetemcomitans Biofilm Reduces Gingival Epithelial Cell Keratin Expression in an Organotypic Gingival Tissue Culture Model [J]. Pathogens, 2019, 8(4): 278.
[13] Kochi S, Yamashiro K, Hongo S, et al. Aggregatibacter actinomycetemcomitans regulates the expression of integrins and reduces cell adhesion via integrin alpha 5 in human gingival epithelial cells [J]. Mol Cell Biochem, 2017, 436(1-2): 39-48.
[14] Zhang L, Koivisto L, Heino J, et al. Bacterial heat shock protein 60 may increase epithelial cell migration through activation of MAP kinases and inhibition of alpha6beta4 integrin expression [J]. Biochem Biophys Res Commun, 2004, 319(4): 1088-95.
[15] Umeda JE, Demuth DR, Ando ES, et al. Signaling transduction analysis in gingival epithelial cells after infection with Aggregatibacter actinomycetemcomitans [J]. Mol Oral Microbiol, 2012, 27(1): 23-33.
[16] Gonzalez-Aparicio M, Alfaro C. Significance of the IL-8 pathway for immunotherapy [J]. Hum Vaccin Immunother, 2020, 16(10): 2312-2317.
[17] Fujita T, Yoshimoto T, Matsuda S, et al. Interleukin-8 induces DNA synthesis, migration and down-regulation of cleaved caspase-3 in cultured human gingival epithelial cells [J]. Journal of Periodontal Research, 2015, 50(4): 479-85.
[18] Pahumunto N, Chotjumlong P, Makeudom A, et al. Pro-inflammatory cytokine responses in human gingival epithelial cells after stimulation with cell wall extract of Aggregatibacter actinomycetemcomitans subtypes [J]. Anaerobe, 2017, 48: 103-109.
[19] Bueno MR, Ishikawa KH, Almeida-Santos G, et al. Lactobacilli Attenuate the Effect of Aggregatibacter actinomycetemcomitans Infection in Gingival Epithelial Cells [J]. Front Microbiol, 2022, 13: 846192.
[20] da Silva MP, Silva VdO, Pasetto S, et al. Aggregatibacter actinomycetemcomitans Outer Membrane Proteins 29 and 29 Paralogue Induce Evasion of Immune Response [J]. Frontiers in oral health, 2022, 3: 835902.
[21] Stolte KN, Pelz C, Yapto CV, et al. IL-1β strengthens the physical barrier in gingival epithelial cells [J]. Tissue Barriers, 2020, 8(3): 1804249.
[22] Tsuruya Y, Yamaguchi A, Yamazaki-Takai M, et al. Interleukin-1β regulates odontogenic ameloblast-associated protein gene transcription in human gingival epithelial cells [J]. Odontology, 2022, 110(3): 557-568.
[23] Hiyoshi T, Domon H, Maekawa T, et al. Aggregatibacter actinomycetemcomitans induces detachment and death of human gingival epithelial cells and fibroblasts via elastase release following leukotoxin-dependent neutrophil lysis [J]. Microbiol Immunol, 2019, 63(3-4): 100-110.
[24] Dommisch H, Jepsen S. Diverse functions of defensins and other antimicrobial peptides in periodontal tissues [J]. Periodontology, 2015, Vol.69(No.1): 96-110.
[25] Niu JY, Yin IX, Mei ML, et al. The multifaceted roles of antimicrobial peptides in oral diseases [J]. Mol Oral Microbiol, 2021, 36(3): 159-171.
[26] Jourdain M-L, Velard F, Pierrard L, et al. Cationic antimicrobial peptides and periodontal physiopathology: A systematic review [J]. J Periodontal Res, 2019, 54(6): 589-600.
[27] Ouhara K, Komatsuzawa H, Shiba H, et al. Actinobacillus actinomycetemcomitans outer membrane protein 100 triggers innate immunity and production of beta-defensin and the 18-kilodalton cationic antimicrobial protein through the fibronectin-integrin pathway in human gingival epithelial cells [J]. Infect Immun, 2006, 74(9): 5211-20.
[28] 刘青青. 伴放线聚集杆菌对人牙龈上皮细胞中β防御素表达的影响 [D]. 广西: 广西医科大学口腔医学院, 2014.
[29] Noguchi T, Shiba H, Komatsuzawa H, et al. Syntheses of prostaglandin E2 and E-cadherin and gene expression of beta-defensin-2 by human gingival epithelial cells in response to Actinobacillus actinomycetemcomitans [J]. Inflammation, 2003, 27(6): 341-9.
[30] Ouhara K, Komatsuzawa H, Yamada S, et al. Susceptibilities of periodontopathogenic and cariogenic bacteria to antibacterial peptides, beta-defensins and LL37, produced by human epithelial cells [J]. J Antimicrob Chemother, 2005, 55(6): 888-96.
[31] Shimada T, Sugano N, Ikeda K, et al. Protease-activated receptor 2 mediates interleukin-8 and intercellular adhesion molecule-1 expression in response to Aggregatibacter actinomycetemcomitans [J]. Oral Microbiol Immunol, 2009, 24(4): 285-91.
[32] Imai H, Fujita T, Kajiya M, et al. Mobilization of TLR4 Into Lipid Rafts by Aggregatibacter Actinomycetemcomitans in Gingival Epithelial Cells [J]. Cell Physiol Biochem, 2016, 39(5): 1777-1786.
[33] Kabir MA, Fujita T, Ouhara K, et al. Houttuynia cordata suppresses the Aggregatibacter actinomycetemcomitans-induced increase of inflammatory-related genes in cultured human gingival epithelial cells(Article) [J]. Journal of Dental Sciences, 2015, 10(1): 88-94.
[34] Miyagawa T, Fujita T, Ouhara K, et al. Irsogladine maleate regulates the inflammatory related genes in human gingival epithelial cells stimulated by Aggregatibacter actinomycetemcomitans [J]. Int Immunopharmacol, 2013, 15(2): 340-7.
[35] Ando-Suguimoto ES, Benakanakere MR, Mayer MPA, et al. Distinct Signaling Pathways Between Human Macrophages and Primary Gingival Epithelial Cells by Aggregatibacter actinomycetemcomitans [J]. Pathogens, 2020, 9(4): 248.
[36] Lu S-Y, Wang C-Y, Jin Y, et al. The osteogenesis-promoting effects of alpha-lipoic acid against glucocorticoid-induced osteoporosis through the NOX4, NF-kappaB, JNK and PI3K/AKT pathways [J]. Sci Rep, 2017, 7(1): 3331.
[37] Xia ZX, Qin L, Tang ZY. Network pharmacology, molecular docking, and experimental pharmacology explored Ermiao wan protected against periodontitis via the PI3K/AKT and NF-κB/MAPK signal pathways [J]. J Ethnopharmacol, 2023, 303: 115900.
[38] Lee BJ, Boyer JA, Burnett GL, et al. Selective inhibitors of mTORC1 activate 4EBP1 and suppress tumor growth [J]. Nat Chem Biol, 2021, 17(10): 1065-1074.
[39] 罗琴, 方梅飞, 刘贞敏, 等. 脂多糖对人牙龈上皮细胞β-防御素表达的影响及其机制研究 [J]. 广西医科大学学报, 2017, 34(02): 173-7
[40] Yoshimoto T, Fujita T, Kajiya M, et al. Aggregatibacter actinomycetemcomitans outer membrane protein 29 (Omp29) induces TGF-β-regulated apoptosis signal in human gingival epithelial cells via fibronectin/integrinβ1/FAK cascade [J]. Cell Microbiol, 2016, 18(12): 1723-1738.
[41] Kajiya M, Komatsuzawa H, Papantonakis A, et al. Aggregatibacter actinomycetemcomitans Omp29 Is Associated with Bacterial Entry to Gingival Epithelial Cells by F-Actin Rearrangement [J]. PLoS One, 2011, 6(4): e18287.
[42] Shenker BJ, Walker LP, Zekavat A, et al. Aggregatibacter actinomycetemcomitans Cytolethal Distending Toxin-Induces Cell Cycle Arrest in a Glycogen Synthase Kinase (GSK)-3-Dependent Manner in Oral Keratinocytes [J]. Int J Mol Sci, 2022, 23(19): 11831.
[43] 黄培勍, 贾小玥, 赵蕾, 等. 牙龈上皮细胞间连接与牙周致病菌关系的研究进展 [J]. 四川大学学报, 2022, 53(02): 214-9.
[44] Tsuyoshi F, Arata A, Hideki S, et al. Regulation of IL-8 by Irsogladine maleate is involved in abolishment of Actinobacillus actinomycetemcomitans-induced reduction of gap-junctional intercellular communication [J]. Cytokine, 2006, 34(5-6): 271-7.
[45] Uchida Y, Shiba H, Komatsuzawa H, et al. Irsogladine maleate influences the response of gap junctional intercellular communication and IL-8 of human gingival epithelial cells following periodontopathogenic bacterial challenge [J]. Biochem Biophys Res Commun, 2005, 333(2): 502-507.
[46] Damek-Poprawa M, Korostoff J, Gill R, et al. Cell Junction Remodeling in Gingival Tissue Exposed to a Microbial Toxin [J]. J Dent Res, 2013, 92(6): 518-523.
[47] Wang PL, Azuma Y, Shinohara M, et al. Effect of Actinobacillus actinomycetemcomitans protease on the proliferation of gingival epithelial cells [J]. Oral Diseases, 2001, 7(4): 233-237.