ANGPTL4通过调节上皮间充质转化促进舌癌细胞转移的分子机制研究

口腔生物医学 ›› 2024, Vol. 15 ›› Issue (1): 32-37.

ANGPTL4通过调节上皮间充质转化促进舌癌细胞转移的分子机制研究

董雨晴¹,张淑宁¹,郭维华¹,张壮²

1. 四川大学华西口腔医学院
2. 华西口腔医院

收稿日期:2023-10-17 修回日期:2023-12-05 出版日期:2024-02-25 发布日期:2024-03-23
通讯作者: 郭维华 E-mail:guoweihua943019@163.com

Molecular mechanism of ANGPTL4 promoting the metastasis of tongue cancer cells by regulating epithelial-mesenchymal transition

Received:2023-10-17 Revised:2023-12-05 Online:2024-02-25 Published:2024-03-23

摘要/Abstract

摘要： 目的：探究血管生成素样蛋白4（ANGPTL4）在舌癌进展及淋巴结转移中的分子机制，以期为舌癌的治疗提供新的分子靶点。方法：使用小干扰RNA（siRNA）沉默Tca8113细胞中的ANGPTL4基因，实时荧光定量PCR检验转染效率，通过CCK-8、Transwell和划痕实验确定ANGPTL4沉默后Tca8113细胞的生物学行为变化。实时荧光定量PCR和Western blot实验检测细胞上皮间充质转化（EMT）相关指标的表达变化。结果：与空白对照组相比，siRNA沉默ANGPTL4后，细胞的增殖显著降低（P＜0.01），且细胞迁移能力明显减弱（P＜0.05）。与EMT相关的钙黏蛋白（Ecadherin）上调，波形蛋白（Vimentin）、盘装结构域受体1（DDR1）、锌指结合蛋白（ZEB-1）的表达下调。结论：沉默ANGPTL4可以降低Tca8113细胞间黏附，促进Tca8113细胞的迁移和EMT，ANGPTL4可为舌癌淋巴结转移的关键靶点。

关键词: ANGPTL4, 舌癌, 增殖和侵袭, 淋巴结转移

Abstract: Objective:?To explore the molecular mechanism of ANGPTL4 in the progression and lymph node metastasis of tongue cancer, in order to provide a new molecular target for the treatment of tongue cancer.. Methods:?The ANGPTL4 gene in tongue cancer cells was silenced by small interfering RNA (siRNA). The transfection efficiency was tested by qRT-PCR. The biological behavior of tongue cancer cells after ANGPTL4 silencing was determined by CCK-8, Transwell and scratch experiments. The expressions of epithelial-to-mesenchymal transition（EMT）related markers were detected by reverse transcription polymerase chain reaction and Western blotting. Results:?After silencing ANGPTL4 by siRNA, the proliferation was significantly reduced （P<0.01） and migration ability of tongue cancer cells was weakened (P＜0.05). The expression of downstream related genes of EMT, E-cadherin was up-regulated and DDR1, Vimentin, ZEB-1 were down-regulated. Conclusions:?Silencing ANGPTL4 can enhance the adhesion between tongue cancer cells, thereby reducing the migration and epithelial-mesenchymal transition of tongue cancer cells. ANGPTL4 may be a key target for lymph node metastasis of tongue cancer.

Key words: ANGPTL4, tongue cancer , proliferation and migration , lymphatic metastasis

董雨晴张淑宁郭维华张壮. ANGPTL4通过调节上皮间充质转化促进舌癌细胞转移的分子机制研究[J]. 口腔生物医学, 2024, 15(1): 32-37.

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