RECQ1沉默对口腔鳞癌细胞增殖、侵袭及上皮间质转化的影响

doi:10.3969/j.issn.1674-8603.2020.02.008

Abstract

Abstract: Objective： To investigate the effects of silencing RECQ1 gene on the proliferation, migration, and epithelial mesenchymal transition (EMT) of oral squamous cell carcinoma. Methods： Human normal oral keratinocytes HOK cells and oral squamous cell carcinoma Cal27 cells were cultured in vitro, and the expressions of RECQ1 mRNA and protein were detected by qRT-PCR and Western Blot. RNAi was used in targeted silence RECQ1 gene in oral squamous cell carcinoma Cal27 cells. The experiment was divided into five groups: si-RECQ1 group (si-RECQ1 transfected into Cal27 cells), si-RECQ1+transforming growth factorβ1 (TGF-β1) group (added TGF-β1 after RECQ1 silence), si-NC group, TGF-β1 group and blank control group.CCK-8 method was used to detect the inhibition rate of cell proliferation in each group. Transwell assay was used to detect the invasive ability of cells. Cell migration ability was measured by cell scratch test. The expressions of RECQ1 and EMT-related proteins, including E-cadherin, N-cadherin and Vimentin, were detected by qRT-PCR and Western Blot. Results： Compared with HOK cells, the expression of RECQ1 mRNA and protein in Cal27 cells increased significantly (P＜0.05); compared with the blank control group and the si-NC group, the expressions of RECQ1 mRNA and protein in Cal27 cells in the si-RECQ1 group decreased significantly (P＜0.05); compared with blank control group, si-NC group and si-RECQ1+TGF-β1 group, the proliferation inhibition rate, E-cadherin mRNA and protein expression levels of Cal27 cells in si-RECQ1 group were increased significantly, while TGF-β1 group decreased significantly (P＜0.05), and Cal27 cell invasion number, migration distance, Vimentin, N-cadherin mRNA and protein expression levels in si-RECQ1 group decreased significantly, but TGF-β1 group increased significantly (P< 0.05). Conclusions： RECQ1 silencing can significantly inhibit the proliferation, migration and invasion of oral squamous cell carcinoma, which may be achieved by inhibiting the EMT process.

Key words: Oral squamous cell carcinoma, RECQ1, Cell proliferation, Cell invasion, Epithelial mesenchymal transition

References 19

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Effects of RECQ1 Silencing on Proliferation, Invasion and Epithelial Mesenchymal Transition of Oral Squamous Cell Carcinoma

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