Abstract: Objective:?To determine the metabolic differences between oral squamous cell carcinoma (OSCC) tissue and the paired adjacent noncancerous tissue by liquid chromatography-mass spectrometry. Methods:Fifteen pairs of OSCC tissues and matched normal tissues were analyzed by liquid chromatography-mass spectrometry. The differential metabolites were screened and determined by principal component analysis and partial least squares discriminant analysis.?Using MetaboAnalyst database to do the metabolic set enrichment analysis and metabolic pathway topological analysis of metabolites for determining abnormal metabolic pathways. Results:? Sixteen different metabolites can we got in OSCC tissues and adjacent tissues after analysis. By metabolic pathway analysis, there may be abnormal metabolic pathways such as sphingolipid metabolism, arginine and proline metabolism, pyrimidine metabolism and glycerophospholipid metabolism in OSCC patients. Conclusions:?There are obvious metabolic abnormalities in the tissues of OSCC and the paired adjacent noncancerous tissue, and abnormal metabolites with diagnostic value can be used as early diagnostic markers for OSCC patients.

Key words: oral squamous cell carcinoma, Metabolomics, UPLC-MS/MS, Differential metabolites