Abstract: Objective：To study the biocompatibility of a new kind of biodegradable poly butylene carbonate (PBC) electrospun film. Methods：Polycaprolactone (PCL) electrospun membranes were prepared as control. Contact angles on the surfaces of two kinds of films were tested. Cell line MC3T3-E1 was seeded onto both kinds of membranes. SEM images were taken to characterize the original morphology of electrospun films and cells one day after seeding. MTT and ALP tests were performed to test the influence of electrospun films on proliferation and differentiation of tested cells. Results：Contact angles in PBC group were lower than those in PCL group, and the difference was significant (P<0.05). One day after seeding, cells attached tightly with both PBC and PCL membranes and spread well on them. There were no significant differences in effects on cell proliferation between two kinds of membranes on the 1st and 7th day (P>0.05), while ALP values in PBC group were significantly higher than those in PCL group on the 7th day (P<0.05). Conclusions：Compared with PCL films, PBC films were a little better in hydrophilicity and cell differentiation, and can be further studied for biomedical use.