Abstract: Objective To construct overexpression vectors targeting rat HDAC8 gene and study the transfection effect of HDAC8 in bone marrow stromal cells ( BMSCs ). Methods The HDAC8 gene was insert into plasmid pGC-FU of lentiviral vector. The plasmid was detected by DNA sequencing. The recombined plasmid pGC-FU-HDAC8 was transfected into 293 cell line. Virus yielded by 293T cell was transfected into rat BMSCs. The expression of HDAC8 was detected by Western bolt. Results The HDAC8 were correctly cloned into the pGC-FU plasmid and confirmed by DNA sequencing. Our result of Western blot showed that pGC-FU-HDAC8 significantly up regulated the expression of HDAC8 in rat BMSCs. Conclusion overexpression vector targeting rat HDAC8 gene had been constructed successfully and efficiently up-regulated the expression of HDAC8 in rat BMSCs.