›› 2014, Vol. 5 ›› Issue (4): 169-173.

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Invitro biologic characteristics of dental follicle cells with cleidocranial dysplasia

  

  • Received:2014-08-29 Revised:2014-12-10 Online:2014-12-25 Published:2015-01-06

Abstract: Objective: To study the invitro biologic characteristics of dental follicle cells with cleidocranial dysplasia (DFCs-CCD) including proliferation ability, clone-forming efficiency, osteogenesis and osteoclastogenesis abilities. Methods: Brdu cell proliferation and population doubling time assays for cell proliferationo ability, crystal violet staining with cells cultured for 12 days for clone-forming efficiency, Western blot and alizarin red staining for osteogenesis ability and real-time PCR for osteoclastogenesis ability. Results: DFCs-CCD gave higher BrdU positive results. The population doubling time was (1.834±0.093) d of DFCs and (1.394±0.028) d of DFCs-CCD (P<0.05). DFCs-CCD presented more clones and weaker expression of Runx2, Osrerix, Ocn and alizarin red staining. DFCs-CCD also expressed stronger levels of OPG and RANK, but no statistical difference of RANKL(P>0.05). Conclusions:DFCs-CCD exhibited stronger cell proliferation and clone-forming abilities, weaker osteogenesis and disorganized osteoclastogenesis abilities.