中药黄芩苷对牙髓干细胞增殖及成牙成骨分化能力的影响

Abstract

Abstract: Objective：To investigate the effect of baicalin on the proliferation and odento/osteogenic differentiation of huaman dental pulp stem cells (hDPSCs) in vitro. Methods：hDPSCs were isolated from dental pulp and then were cultured in alpha minimun essential medium(α-MEM) supplimented with or without baicalin. MTT colorimetric method was used to examine the proliferation ability of hDPSCs. The effect of baicalin on the odento/osteogenic differentiation was observed by alkaline phosphotase(ALP) activity and Western blot. Results： MTT assay showed that there was no significant difference between baicalin-treated and untreated control cells (P>0.05), and ALP activity assay demonstrated that 0~20μmol/L baicalin enhanced the ALP activity of DPSCs(P<0.01), and Western blot revealed that the odento/osteogenic marker (RUNX2, DSP, OSX, OPN, OCN) was significantly upregulated in baicalin-treated cells compared with the untreated cells. Conclusions： Baicalin has no effect on the proliferation of DPSCs, but enhances the capacity of odento/osteogenic differentiation.

CLC Number:

R780.2

References

[1]Gronthos S1, Mankani M, Brahim J, et al.Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo[J].Proc Natl Acad Sci U S A,2000,97(25):13625-13630.
[2]Xu M1, Chen X, Gu Y, et al.Baicalin can scavenge peroxynitrite and ameliorate endogenous peroxynitrite-mediated neurotoxicity in cerebral ischemia-reperfusion injury[J].J Ethnopharmacol,2013,150(1):116-124.
[3]雷蕾, 谭为霞, 吴堪葵, 等.黄芩苷在急性深龋盖髓术中的应用[J].暨南大学学报(自然科学与医学版),2008,29(4):401-402.
[4]钟波, 雷蕾, 于淼, 等.黄芩苷在可复性牙髓炎盖髓术中的应用[J].口腔临床医学杂志,2009,25(5):310-311.
[5]Vandomme J1, Touil Y, Ostyn P, et al.Insulin-like growth factor 1 receptor and p38 mitogen-activated protein kinase signals inversely regulate signal transducer and activator of transcription 3 activity to control human dental pulp stem cell quiescence, propagation, and differentiation[J].Stem Cells Dev,2014,23(8):839-851.
[6]Zhang Z1, Guo Q1, Tian H1, et al.Effects of WNT10A on proliferation and differentiation of human dental pulp cells[J].J Endod,2014,40(10):1593-1599.
[7]Horst OV1, Horst JA, Samudrala R, et al.Caries induced cytokine network in the odontoblast layer of human teeth[J].BMC Immunol,2011,12:9-.
[8]Lee W1, Ku SK, Bae JS.Anti-inflammatory effects of Baicalin, Baicalein, and Wogonin in vitro and in vivo[J].Inflammation,2015,38(1):110-125.
[9]Luo W1, Wang CY, Jin L.Baicalin downregulates Porphyromonas gingivalis lipopolysaccharide-upregulated IL-6 and IL-8 expression in human oral keratinocytes by negative regulation of TLR signaling[J].PLoS One,2012,7(12):e51008-.
[10]于淼, 雷蕾, 谭为霞, 等.黄芩苷对LPS诱导人牙髓细胞TLR4表达及TNF-α分泌的影响[J].牙体牙髓牙周病学杂志,2009,19(11):632-636.
[11]Qiao H1, Tong Y, Han H, et al.A novel therapeutic regimen for hepatic fibrosis using the combination of mesenchymal stem cells and baicalin[J].Pharmazie,2011,66(1):37-43.
[12]Shin SH1, Bak SS, Kim MKet al.Baicalin, a flavonoid, affects the activity of human dermal papilla cells and promotes anagen induction in mice[J].Naunyn Schmiedebergs Arch Pharmacol,2014,http://link.springer.com/article/10.1007%2Fs00210-014-1075-0#:-.
[13]Wang GF1, Wu ZF, Wan L, et al.Influence of baicalin on the expression of receptor activator of nuclear factor-kappaB ligand in cultured human periodontal ligament cells[J].Pharmacology,2006,77(2):71-77.
[14]李昂, 赵俊杰, 郭宁, 等.黄芩苷对人牙周膜细胞功能活性调节的实验研究[J].口腔生物医学,2011,2(2):62-66.
[15]Shiba H1, Nakanishi K, Rashid F, et al.Proliferative ability and alkaline phosphatase activity with in vivo cellular aging in human pulp cells[J].J Endod,2003,29(1):9-11.

Effect of baicalin on the proliferation and odento/osteogenic differentiation of human dental pulp stem cells

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