Abstract: Objective：To observe the effects of endotoxin tolerance on the production of chemokine epithelial neutrophil-activating peptide 78 (ENA-78) and epidermal growth factor (EGF) in THP-1 cells. Methods：THP-1 cells were pretreated with 1μg/ml Porphyromonas gingivalis (P.gingivalis) lipopolysaccharides (LPS) or 1μg/ml Escherichia coli (E.coli) LPS (24h), washed and stimulated with the same LPS again (24h). Levels of ENA-78 and EGF in supernatants were detected by ELISA. Results：There were no differences in production of ENA-78 secreted by THP-1 cells stimulated with P.gingivalis LPS and culture medium (p>0.05), while the amounts of ENA-78 in the cells challenged by E.coli LPS were increased significantly compared with those without any stimulations (p<0.05). In addition, secretions of ENA-78 were decreased significantly after repeated P.gingivalis LPS or E.coli LPS stimulations compared with those following signal challenge (p<0.05). However, no significant changes of EGF levels were detected in THP-1 cells restimulated with P.gingivalis LPS or E.coli LPS compared with those challenged only once (p>0.05). Conclusions：Secretions of ENA-78 in THP-1 cells could be suppressed by endotoxin tolerance, which might have an effect on neutrophil migration.