Abstract: [Abstract] Objective： To evaluate the effect of curcumin on biofilm formation by Enterococus faecalis. Methods： The dilution method was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Biofilms were formed after incubating at 37 ℃ for 24 h with different concentrations of curcumin, MTT assay method was used to compare the biofilm formation quantificationally. Confocal laser scanning microscope (CLSM) was used to observe the biofilm structure after treated with different concentrations of curcumin. Results：The MIC and MBC of curcumin on Enterococus faecalis were of 64 μg/mL and 256 μg/mL respectively. The quantity of biofilm formed by Enterococus faecalis significantly decreased (P<0.05) and the structure of the biofilm shown by CLSM became thinner with the increase of curcumin concentration. Conclusions： Curcumin can inhibit the biofilm formation of Enterococus faecalis.