Abstract: Objective：To investigate the effect of porous TiO2 modified titanium implant prepared by micro-arc oxidation on the adhesion and osteogenic differentiation in bone marrow mesenchymal stem cells (BMSCs). Methods：Micro-arc oxidation technology was used to prepare the coating on the pure titanium surface. The surface morphology and crystal phase of the coating were detected by SEM and XRD. After co-culture of material and BMSCs, the expression of adhesion related protein integrin β1 was detected by immunofluorescence staining, in addition, the cytotoxicity of the material was detected by living dead cell staining and MTT. Alkaline phosphatase (ALP) and alizarin red S (ARS) staining assay and RT-PCR were used to evaluate osteogenic differentiation of the BMSCs. Results：The coating of the MAO group treated by micro arc oxidation was microporous TiO2, moreover, the coating not only had no obvious cytotoxicity, but also promoted the expression of integrin β1 in BMSCs. The results showed that both ALP and ARS staining and quantitative of the MAO group was significantly higher than the Ti group which was the untreated group (P<0.05). The results of RT-PCR showed that the expression level of BMSCs ALP and OSX was significantly increased in MAO group over time. Conclusions：The porous TiO2 coating prepared by micro-arc oxidation on pure titanium surface enhanced adhesion and osteogenic differentiation of BMSCs.