›› 2018, Vol. 9 ›› Issue (3): 120-124.

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SFRP2 promoted osteogenic differentiation of adipose-derived stem cells

Hao-Qing YANG1, 2   

  1. 1. ,Capital Medical University School of Stomatology
    2. Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical University
  • Received:2018-03-27 Revised:2018-07-12 Online:2018-09-25 Published:2018-09-29
  • Supported by:
    Beijing Natural Science Foundation

Abstract: Objective:To investigate the effect of SFRP2 on osteogenic differentiation potential of adipose-derived stem cells (ADSC). Methods:Lentiviral SFRP2 shRNA was used to silence the expression of SFRP2. Retroviral HA-SFRP2 was used to over-express the SFRP2 in ADSC; real-time RT-PCR and Western Blot were used to detect the expression of SFRP2. Alkaline phosphatase activity, alizarin red staining, mineralization and real-time RT-PCR were used to detect the osteogenic differentiation of adipose-derived stem cells. Results:Real-time RT-PCR and Western Blot results showed that SFRP2sh effectively reduced the expression of SFRP2 in ADSC. Depletion of SFRP2 reduced alkaline phosphatase, mineralization and bone sialoprotein (BSP), Osterix (Osx) and runt-related transcription factor-2 (RUNX2) in ADSC. Real-time RT-PCR and Western Blot showed that HA-SFRP2 can be effectively overexpressed in ADSC. The results of alkaline phosphatase, alizarin red and calcium qualitative analysis results showed that the over expressed SFRP2 in ADSC could significantly promote the osteogenic differentiation of ADSC. Real-time RT-PCR results showed that the SFRP2 significantly promoted the expression of BSP. Conclusions:SFRP2 can promote osteogenic differentiation of adipose stem cells and prove that SFRP2 is the key gene for osteogenic differentiation of ADSC.