Abstract: Objective： To investigate the effect of local application of resolvin E1 (RvE1) on periodontal inflammation in mice and its regulation of periodontal ligament cells. Methods： C57BL/6 mice were randomly divided into normal group, control group (periodontitis + PBS) and RvE1 group (periodontitis + RvE1). Silk ligation was used to construct periodontitis models on mice, RvE1 was locally injected every other day for 10 days in all. The inflammation changes in periodontium was compared. And the expression of ChemR23 was checked. Human periodontal ligament cells(hPDLCs) were isolated and cultured in vitro. 1 μg/mL lipopolysaccharide was added to culture medium to induce the inflammation condition. Different concentration of RvE1 was added, and then the expression of inflammatory factors, IL–1β, IL-6 and IL-8 was detected. Results： The inflammation infiltration was depressed with the local injections of RvE1. PDLCs express ChemR23 receptor around the nuclei in normal and inflammatory state. 1 nmol/L RvE1 suppressed the expression of IL–1β, IL-6 and IL-8 in periodontal ligament cells at RNA and protein levels. Conclusions： RvE1 inhibits the expression of inflammatory cytokines in periodontal ligament cells and suppresses the inflammatory status of periodontal tissues.

Key words: Resolvin E1, Periodontal ligament cells, Periodontitis